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pubmed-article:11265628pubmed:dateCreated2001-3-26lld:pubmed
pubmed-article:11265628pubmed:abstractTextA new method of culture of peripheral blood leukocytes (PBMC) from HIV+ patients, in the absence of exogenous stimuli (allogeneic cells or cytokines) (PBMC w/s) was used for the detection of persistent viral infection in HIV patients who had undergone successful highly active antiretroviral therapy (HAART) lowering their viral burden to undetectable levels (< 50 RNA copies/ml). Infected cells were always of the monocyte/macrophage lineage (M). No infection could be detected in these patients using the classical system (co-culture with HIV-CMP activated with PHA and IL-2). Differences in the class of target cells (higher proportion of proliferating M and CCR5 expressing cells in the PBMC w/s system than in PBMC-PHA cultures) may determine the relative sensitivity of each technique to achieve successful isolation of HIV from different patients.lld:pubmed
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pubmed-article:11265628pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
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pubmed-article:11265628pubmed:statusMEDLINElld:pubmed
pubmed-article:11265628pubmed:issn0025-7680lld:pubmed
pubmed-article:11265628pubmed:authorpubmed-author:Ruibal-AresBBlld:pubmed
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pubmed-article:11265628pubmed:authorpubmed-author:BelmonteLLlld:pubmed
pubmed-article:11265628pubmed:issnTypePrintlld:pubmed
pubmed-article:11265628pubmed:volume61lld:pubmed
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pubmed-article:11265628pubmed:pagination73-5lld:pubmed
pubmed-article:11265628pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11265628pubmed:year2001lld:pubmed
pubmed-article:11265628pubmed:articleTitle[HIV infected macrophages isolated from HIV+ patients with undetectable viral load undergoing combined antiretroviral treatment].lld:pubmed
pubmed-article:11265628pubmed:affiliationInstituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 Buenos Aires, Argentina. direccion@iihema.anm.edu.arlld:pubmed
pubmed-article:11265628pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11265628pubmed:publicationTypeEnglish Abstractlld:pubmed