| pubmed-article:11239009 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0162775 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C1444754 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0547040 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C0039315 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C1556066 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C1619636 | lld:lifeskim |
| pubmed-article:11239009 | lifeskim:mentions | umls-concept:C1514873 | lld:lifeskim |
| pubmed-article:11239009 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11239009 | pubmed:dateCreated | 2001-3-12 | lld:pubmed |
| pubmed-article:11239009 | pubmed:abstractText | The transformation-associated recombination (TAR) cloning technique allows selective and accurate isolation of chromosomal regions and genes from complex genomes. The technique is based on in vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or hooks, to the gene of interest. The recombination occurs during transformation of yeast spheroplasts that results in the generation of a yeast artificial chromosome (YAC) containing the gene of interest. To further enhance and refine the TAR cloning technology, we determined the minimal size of a specific hook required for gene isolation utilizing the Tg.AC mouse transgene as a targeted region. For this purpose a set of vectors containing a B1 repeat hook and a Tg.AC-specific hook of variable sizes (from 20 to 800 bp) was constructed and checked for efficiency of transgene isolation by a radial TAR cloning. When vectors with a specific hook that was >/=60 bp were utilized, approximately 2% of transformants contained circular YACs with the Tg.AC transgene sequences. Efficiency of cloning dramatically decreased when the TAR vector contained a hook of 40 bp or less. Thus, the minimal length of a unique sequence required for gene isolation by TAR is approximately 60 bp. No transgene-positive YAC clones were detected when an ARS element was incorporated into a vector, demonstrating that the absence of a yeast origin of replication in a vector is a prerequisite for efficient gene isolation by TAR cloning. | lld:pubmed |
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| pubmed-article:11239009 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11239009 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11239009 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11239009 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11239009 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11239009 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11239009 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11239009 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:BarrettJ CJC | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:RandolphMM | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:StubbsLL | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:SolomonGG | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:KouprinaNN | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:LeemS HSH | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:NoskovV NVN | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:LarionovVV | lld:pubmed |
| pubmed-article:11239009 | pubmed:author | pubmed-author:KoriabineMM | lld:pubmed |
| pubmed-article:11239009 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:11239009 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11239009 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:11239009 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11239009 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11239009 | pubmed:pagination | E32 | lld:pubmed |
| pubmed-article:11239009 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:meshHeading | pubmed-meshheading:11239009... | lld:pubmed |
| pubmed-article:11239009 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11239009 | pubmed:articleTitle | Defining the minimal length of sequence homology required for selective gene isolation by TAR cloning. | lld:pubmed |
| pubmed-article:11239009 | pubmed:affiliation | Laboratory of Molecular Genetics and Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. | lld:pubmed |
| pubmed-article:11239009 | pubmed:publicationType | Journal Article | lld:pubmed |
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