| pubmed-article:11216004 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C0005768 | lld:lifeskim |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C0178913 | lld:lifeskim |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C0577559 | lld:lifeskim |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:11216004 | lifeskim:mentions | umls-concept:C0205266 | lld:lifeskim |
| pubmed-article:11216004 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11216004 | pubmed:dateCreated | 2001-2-16 | lld:pubmed |
| pubmed-article:11216004 | pubmed:abstractText | An analytical method was developed for the quantitation of intact insulin in blood samples. Solid-phase extraction (SPE) was used to purify and concentrate the protein after the plasma is separated. Analysis is performed by electrospray liquid chromatography-mass spectrometry (LC-MS) using a trifluoroacetic acid mobile phase. The limit of quantitation of the SPE LC-MS method has been determined to be 1.0 ng/mL for endogenous levels of insulin. Base levels of human insulin in plasma have been quantitated, and values ranging from 1.0 to 1.4 ng/mL were observed. In a single analysis, the method can determine human, porcine, and bovine insulin. Reproducibility was tested for both blood samples and aqueous standards and produced relative standard deviations of approximately 10% and lower. Calibration curves were constructed corresponding to plasma levels of 0.4 to 80 ng/mL and found to be linear with R2 values greater than 0.99. Stability studies of human and porcine insulin were performed over a period of 21 days for whole human blood samples stored at both room temperature and 4 degrees C. Hemolyzed blood samples were also analyzed using the developed method and were found to produce quantitatable levels of insulin. The advantage of the application of SPE and LC-MS for the quantitation of insulin is the high specificity compared to other techniques such as radioimmunoassay (RIA). In addition, the developed LC-MS method is not subject to interferences that cause problems with RIA, such as hemolysis. The method is efficient and rapid and produces results more specific than those obtained with RIA. | lld:pubmed |
| pubmed-article:11216004 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11216004 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11216004 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11216004 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11216004 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11216004 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11216004 | pubmed:issn | 0146-4760 | lld:pubmed |
| pubmed-article:11216004 | pubmed:author | pubmed-author:MillerM LML | lld:pubmed |
| pubmed-article:11216004 | pubmed:author | pubmed-author:AllenR ORO | lld:pubmed |
| pubmed-article:11216004 | pubmed:author | pubmed-author:LeBeauMM | lld:pubmed |
| pubmed-article:11216004 | pubmed:author | pubmed-author:DarbyS MSM | lld:pubmed |
| pubmed-article:11216004 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11216004 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:11216004 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11216004 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11216004 | pubmed:pagination | 8-14 | lld:pubmed |
| pubmed-article:11216004 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:meshHeading | pubmed-meshheading:11216004... | lld:pubmed |
| pubmed-article:11216004 | pubmed:articleTitle | A mass spectrometric method for quantitation of intact insulin in blood samples. | lld:pubmed |
| pubmed-article:11216004 | pubmed:affiliation | University of Virginia, Department of Chemistry, Charlottesville, Virginia 22903, USA. | lld:pubmed |
| pubmed-article:11216004 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11216004 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11216004 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11216004 | lld:pubmed |
