| pubmed-article:11171999 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0074129 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0008387 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C1413218 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0456387 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11171999 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11171999 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11171999 | pubmed:dateCreated | 2001-2-26 | lld:pubmed |
| pubmed-article:11171999 | pubmed:abstractText | In the "selective" cholesteryl ester (CE) uptake process, surface-associated lipoproteins [high density lipoprotein (HDL) and low density lipoprotein] are trapped in the space formed between closely apposed surface microvilli (microvillar channels) in hormone-stimulated steroidogenic cells. This is the same location where an HDL receptor (SR-BI) is found. In the current study, we sought to understand the relationship between SR-BI and selective CE uptake in a heterologous insect cell system. Sf9 (Spodoptera frugiperda) cells overexpressing recombinant SR-BI were examined for (i) SR-BI protein by Western blot analysis and light or electron immunomicroscopy, and (ii) selective lipoprotein CE uptake by the use of radiolabeled or fluorescent (BODIPY-CE)-labeled HDL. Noninfected or infected control Sf9 cells do not express SR-BI, show microvillar channels, or internalize CEs. An unexpected finding was the induction of a complex channel system in Sf9 cells expressing SR-BI. SR-BI-expressing cells showed many cell surface double-membraned channels, immunogold SR-BI, apolipoprotein (HDL) labeling of the channels, and high levels of selective HDL-CE uptake. Thus, double-membraned channels can be induced by expression of recombinant SR-BI in a heterologous system, and these specialized structures facilitate both the binding of HDL and selective HDL-CE uptake. | lld:pubmed |
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| pubmed-article:11171999 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11171999 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11171999 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11171999 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11171999 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11171999 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:11171999 | pubmed:author | pubmed-author:AzharSS | lld:pubmed |
| pubmed-article:11171999 | pubmed:author | pubmed-author:NomotoAA | lld:pubmed |
| pubmed-article:11171999 | pubmed:author | pubmed-author:ReavenEE | lld:pubmed |
| pubmed-article:11171999 | pubmed:author | pubmed-author:Leers-Sucheta... | lld:pubmed |
| pubmed-article:11171999 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11171999 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:11171999 | pubmed:volume | 98 | lld:pubmed |
| pubmed-article:11171999 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11171999 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11171999 | pubmed:pagination | 1613-8 | lld:pubmed |
| pubmed-article:11171999 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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