| pubmed-article:11158282 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11158282 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:11158282 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:11158282 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:11158282 | lifeskim:mentions | umls-concept:C0014239 | lld:lifeskim |
| pubmed-article:11158282 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:11158282 | pubmed:issue | Pt 3 | lld:pubmed |
| pubmed-article:11158282 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11158282 | pubmed:abstractText | The regulation of organelle free Ca2+ was analysed in individual mouse pancreatic beta-cells loaded with the fluorescent low-affinity indicator furaptra. Removal of the cytoplasmic indicator by controlled digitonin permeabilization of the plasma membrane resulted in a sudden increase of the 340 nm/380 nm fluorescence excitation ratio followed by a gradual decay, reflecting the emptying of Ca2+ from organelle pools. Subsequent introduction of 3 mM ATP caused rapid refilling of a Ca2+ pool, which represented the endoplasmic reticulum (ER) in being mobilized with inositol 1,4,5-trisphosphate (IP3) and the sarco(endo)plasmic reticulum Ca2+-ATPase inhibitor thapsigargin. The concentration of Ca2+ in the ER observed immediately after permeabilization depended on the glucose concentration in a hyperbolic fashion with half-maximal filling at about 6 mM of the sugar. Glucose promotion of Ca2+ sequestration in the ER involved a high-affinity mechanism not requiring but accelerated by a rise of the cytoplasmic Ca2+ concentration. Glucose also exerted a long-term action on the ER storage of Ca2+, maintaining the set-point for its maximal concentration and preserving the response to IP3. The results indicate that the ER has an important role in the glucose-stimulated beta-cell by serving as a high-affinity sink for Ca2+, irrespective of the prevailing concentration of cytoplasmic Ca2+. | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11158282 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158282 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11158282 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11158282 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:11158282 | pubmed:author | pubmed-author:HellmanBB | lld:pubmed |
| pubmed-article:11158282 | pubmed:author | pubmed-author:GylfeEE | lld:pubmed |
| pubmed-article:11158282 | pubmed:author | pubmed-author:TengholmAA | lld:pubmed |
| pubmed-article:11158282 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11158282 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11158282 | pubmed:volume | 530 | lld:pubmed |
| pubmed-article:11158282 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11158282 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11158282 | pubmed:pagination | 533-40 | lld:pubmed |
| pubmed-article:11158282 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:meshHeading | pubmed-meshheading:11158282... | lld:pubmed |
| pubmed-article:11158282 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11158282 | pubmed:articleTitle | The endoplasmic reticulum is a glucose-modulated high-affinity sink for Ca2+ in mouse pancreatic beta-cells. | lld:pubmed |
| pubmed-article:11158282 | pubmed:affiliation | Department of Medical Cell Biology, Uppsala University, Biomedical Centre, Box 571, SE-751 23 Uppsala, Sweden. | lld:pubmed |
| pubmed-article:11158282 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11158282 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11158282 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11158282 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11158282 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11158282 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11158282 | lld:pubmed |
