| pubmed-article:11158091 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11158091 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:11158091 | lifeskim:mentions | umls-concept:C0150369 | lld:lifeskim |
| pubmed-article:11158091 | lifeskim:mentions | umls-concept:C0033607 | lld:lifeskim |
| pubmed-article:11158091 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:11158091 | lifeskim:mentions | umls-concept:C2737269 | lld:lifeskim |
| pubmed-article:11158091 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11158091 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11158091 | pubmed:abstractText | The emergence of drug-resistant viral variants is the inevitable consequence of incomplete suppression of human immunodeficiency virus type 1 (HIV-1) replication during treatment with antiretroviral drugs. Sequencing to determine the resistance profiles of these variants has become increasingly important in the clinical management of HIV-1 patients, both in the initial design of a therapeutic plan and in selecting a salvage regimen. Here we have developed a pyrosequencing assay for the rapid characterization of resistance to HIV-1 protease inhibitors (PIs). Twelve pyrosequencing primers were designed and were evaluated on the MN strain and on viral DNA from peripheral blood mononuclear cells from eight untreated HIV-1-infected individuals. The method had a limit of detection of 20 to 25% for minor sequence variants. Pattern recognition (i.e., comparing actual sequence data with expected wild-type and mutant sequence patterns) simplified the identification of minor sequence variants. This real-time pyrosequencing method was applied in a longitudinal study monitoring the development of PI resistance in plasma samples obtained from four patients over a 2 1/2-year period. Pyrosequencing identified eight primary PI resistance mutations as well as several secondary mutations. This sequencing approach allows parallel analysis of 96 reactions in 1 h, facilitating the monitoring of drug resistance in eight patients simultaneously and, in combination with viral load analysis, should be a useful tool in the future to monitor HIV-1 during therapy. | lld:pubmed |
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| pubmed-article:11158091 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158091 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11158091 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11158091 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158091 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11158091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11158091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11158091 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11158091 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11158091 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:11158091 | pubmed:author | pubmed-author:AlbertJJ | lld:pubmed |
| pubmed-article:11158091 | pubmed:author | pubmed-author:O'MearaDD | lld:pubmed |
| pubmed-article:11158091 | pubmed:author | pubmed-author:LundebergJJ | lld:pubmed |
| pubmed-article:11158091 | pubmed:author | pubmed-author:LeitnerTT | lld:pubmed |
| pubmed-article:11158091 | pubmed:author | pubmed-author:HejdemanBB | lld:pubmed |
| pubmed-article:11158091 | pubmed:author | pubmed-author:WilbeKK | lld:pubmed |
| pubmed-article:11158091 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11158091 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:11158091 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11158091 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11158091 | pubmed:pagination | 464-73 | lld:pubmed |
| pubmed-article:11158091 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11158091 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11158091 | pubmed:articleTitle | Monitoring resistance to human immunodeficiency virus type 1 protease inhibitors by pyrosequencing. | lld:pubmed |
| pubmed-article:11158091 | pubmed:affiliation | Department of Biotechnology, Royal Institute of Technology (KTH), S-100 44 Stockholm, Sweden. | lld:pubmed |
| pubmed-article:11158091 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11158091 | pubmed:publicationType | Case Reports | lld:pubmed |
| pubmed-article:11158091 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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