| pubmed-article:11157940 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C0040711 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C0242610 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C0001721 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C1323391 | lld:lifeskim |
| pubmed-article:11157940 | lifeskim:mentions | umls-concept:C2242140 | lld:lifeskim |
| pubmed-article:11157940 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11157940 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11157940 | pubmed:abstractText | Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above background levels. Addition of an untranslated lac leader and Shine-Dalgarno sequence to cI increased expression but still showed a dependence on an AUG for maximum expression. cI-lacZ mRNA with an AUG initiation codon showed a greater in vitro ribosome binding strength and a higher level of full-length in vivo mRNA, suggesting that the initiation codon is an important determinant of ribosome binding strength and translational efficiency for mRNA with or without the 5' untranslated leader. | lld:pubmed |
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| pubmed-article:11157940 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:11157940 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11157940 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11157940 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11157940 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:11157940 | pubmed:author | pubmed-author:JanssenG RGR | lld:pubmed |
| pubmed-article:11157940 | pubmed:author | pubmed-author:O'DonnellS... | lld:pubmed |
| pubmed-article:11157940 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11157940 | pubmed:volume | 183 | lld:pubmed |
| pubmed-article:11157940 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11157940 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11157940 | pubmed:pagination | 1277-83 | lld:pubmed |
| pubmed-article:11157940 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11157940 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11157940 | pubmed:articleTitle | The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader. | lld:pubmed |
| pubmed-article:11157940 | pubmed:affiliation | Department of Microbiology, Miami University, Oxford, Ohio 45056, USA. | lld:pubmed |
| pubmed-article:11157940 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11157940 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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