| pubmed-article:11157849 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11157849 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:11157849 | lifeskim:mentions | umls-concept:C1515432 | lld:lifeskim |
| pubmed-article:11157849 | lifeskim:mentions | umls-concept:C1332709 | lld:lifeskim |
| pubmed-article:11157849 | lifeskim:mentions | umls-concept:C1413236 | lld:lifeskim |
| pubmed-article:11157849 | lifeskim:mentions | umls-concept:C1327413 | lld:lifeskim |
| pubmed-article:11157849 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:11157849 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:11157849 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11157849 | pubmed:abstractText | CD7 and CD28 are Ig superfamily molecules expressed on thymocytes and mature T cells that share common signaling 0mechanisms and are co-mitogens for T cell activation. CD7-deficient mice are resistant to lipopolysaccharide (LPS)-induced shock syndrome, and have diminished in vivo LPS-triggered IFN-gamma and tumor necrosis factor (TNF)-alpha production. CD28-deficient mice have decreased serum Ig levels, defective IgG isotype switching, decreased T cell IL-2 production and are resistant to Staphylococcus aureus enterotoxin-induced shock. To determine synergistic roles CD7 and CD28 might play in thymocyte development and function, we have generated and characterized CD7/CD28 double-deficient mice. CD7/CD28-deficient mice were healthy, reproduced normally, had normal numbers of thymocyte subsets and had normal thymus histology. Anti-CD3 mAb induced similar levels of apoptosis in CD7-deficient, CD28-deficient and CD7/CD28 double-deficient thymocytes as in control C57BL/6 mice (P = NS). Similarly, thymocyte viability, apoptosis and necrosis following ionomycin or dexamethasone treatment were the same in control, CD7-deficient, CD28-deficient and CD7/CD28-deficient mice. CD28-deficient and CD7/CD28-deficient thymocytes had decreased [3H]thymidine incorporation responses to concanavalin A (Con A) stimulation compared to control mice (P < or = 0.01 and P < or = 0.05 respectively). CD7/CD28 double-deficient mice had significantly reduced numbers of B7-1/B7-2 double-positive cells compared to freshly isolated wild-type, CD7-deficient and CD28-deficient thymocytes. Con A-stimulated CD4/CD8 double-negative (DN) thymocytes from CD7/CD28 double-deficient mice expressed significantly lower levels of CD25 when compared to CD4/CD8 DN thymocytes from wild-type, CD7-deficient and CD28-deficient mice (P < 0.05). Anti-CD3-triggered CD7/CD28-deficient thymocytes also had decreased IFN-gamma and TNF-alpha production compared to C57BL/6 control, CD7-deficient and CD28-deficient mice (P < or = 0.05). Thus, CD7 and CD28 deficiencies combined to produce abnormalities in the absolute number of B7-1/B7-2-expressing cells in the thymus, thymocyte IL-2 receptor expression and CD3-triggered cytokine production. | lld:pubmed |
| pubmed-article:11157849 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11157849 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11157849 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11157849 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11157849 | pubmed:issn | 0953-8178 | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:HaynesB FBF | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:LeeD MDM | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:ThompsonC BCB | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:PatelD DDD | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:SempowskiG... | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:ScearceR MRM | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:HeinlyC SCS | lld:pubmed |
| pubmed-article:11157849 | pubmed:author | pubmed-author:McDermottP... | lld:pubmed |
| pubmed-article:11157849 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11157849 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:11157849 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11157849 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11157849 | pubmed:pagination | 157-66 | lld:pubmed |
| pubmed-article:11157849 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:11157849 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11157849 | pubmed:articleTitle | Comparison of thymocyte development and cytokine production in CD7-deficient, CD28-deficient and CD7/CD28 double-deficient mice. | lld:pubmed |
| pubmed-article:11157849 | pubmed:affiliation | Division of Rheumatology, Allergy and Clinical Immunology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA. | lld:pubmed |
| pubmed-article:11157849 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11157849 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:11157849 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| entrez-gene:12487 | entrezgene:pubmed | pubmed-article:11157849 | lld:entrezgene |
| entrez-gene:12516 | entrezgene:pubmed | pubmed-article:11157849 | lld:entrezgene |
