| pubmed-article:11147794 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11147794 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:11147794 | lifeskim:mentions | umls-concept:C1258666 | lld:lifeskim |
| pubmed-article:11147794 | lifeskim:mentions | umls-concept:C1256369 | lld:lifeskim |
| pubmed-article:11147794 | lifeskim:mentions | umls-concept:C2346753 | lld:lifeskim |
| pubmed-article:11147794 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:11147794 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11147794 | pubmed:dateCreated | 2001-1-5 | lld:pubmed |
| pubmed-article:11147794 | pubmed:abstractText | Neuron activity and insulin release were measured simultaneously from 33 preparations of intrapancreatic canine ganglia and pancreatic parenchyma adjacent to the ganglia. The electrical activity of single neurons of the ganglia was recorded with intracellular microelectrodes, and insulin release from the attached islets was determined with an enzyme-linked immunosorbent assay. Insulin release was 62 +/- 18 fmol preparation/min in the presence of 10 mmol/l glucose and pulsatile (3.7 +/- 0.4 min/pulse). Corresponding measurements of neuronal electrical activity showed a stable membrane potential of -53.5 +/- 0.6 mV. Short, high-frequency (20 Hz) preganglionic nerve stimulation evoked action potentials and, in 46% of the preparations, a threefold rise in the insulin secretory rate associated with increased amplitude of the insulin pulses. The effects were blocked by 10 micromol/l tetrodotoxin (TTX). In other preparations, continuous low-frequency (0.05-0.5 Hz) preganglionic nerve stimulation evoked action potentials and, in 50% of the preparations, a gradual increase of insulin release associated with augmentation of insulin pulse amplitude without alteration of the duration. The effects were blocked by 50 micromol/l hexamethonium (HEX). In the remaining preparations, no change in insulin release was observed during nerve stimulation. In the absence of stimulation, neither TTX nor HEX affected the membrane potential or insulin secretion. These first simultaneous measurements of intrapancreatic ganglion activity and insulin secretion are consistent with amplitude modulation of pulsatile insulin secretion induced by changes in electrical activity in a population of intrapancreatic ganglion neurons. | lld:pubmed |
| pubmed-article:11147794 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11147794 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11147794 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11147794 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:11147794 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11147794 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11147794 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11147794 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11147794 | pubmed:issn | 0012-1797 | lld:pubmed |
| pubmed-article:11147794 | pubmed:author | pubmed-author:SzurszewskiJ... | lld:pubmed |
| pubmed-article:11147794 | pubmed:author | pubmed-author:ShiTT | lld:pubmed |
| pubmed-article:11147794 | pubmed:author | pubmed-author:WesterlundJJ | lld:pubmed |
| pubmed-article:11147794 | pubmed:author | pubmed-author:BergstenPP | lld:pubmed |
| pubmed-article:11147794 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11147794 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:11147794 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11147794 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11147794 | pubmed:pagination | 51-5 | lld:pubmed |
| pubmed-article:11147794 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:11147794 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11147794 | pubmed:articleTitle | Amplitude modulation of pulsatile insulin secretion by intrapancreatic ganglion neurons. | lld:pubmed |
| pubmed-article:11147794 | pubmed:affiliation | Department of Physiology and Biophysics, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, USA. | lld:pubmed |
| pubmed-article:11147794 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11147794 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11147794 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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