| pubmed-article:11135552 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11135552 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:11135552 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
| pubmed-article:11135552 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:11135552 | lifeskim:mentions | umls-concept:C0597731 | lld:lifeskim |
| pubmed-article:11135552 | lifeskim:mentions | umls-concept:C0441633 | lld:lifeskim |
| pubmed-article:11135552 | lifeskim:mentions | umls-concept:C1881865 | lld:lifeskim |
| pubmed-article:11135552 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11135552 | pubmed:dateCreated | 2001-2-12 | lld:pubmed |
| pubmed-article:11135552 | pubmed:abstractText | Here we have designed and synthesized ligands that specifically bind with high affinity (K(d) = 53 nM) to the guanine (G)-guanine mismatch, one of four types of single-nucleotide polymorphism (SNP). Detection of the G-G mismatch was performed by a surface plasmon resonance (SPR) assay using a sensor chip carrying the G-G specific ligand on its surface. The accuracy of the G-G mismatch detection by the SPR sensor was demonstrated by a marked SPR response obtained only for the DNA containing the G-G mismatch. DNAs containing G-A and G-T mismatches, as well as a fully matched duplex, produced only a weak response. Furthermore, this assay was found applicable for the detection of SNP existing in PCR amplification products of a 652-nucleotide sequence of the HSP70-2 gene. | lld:pubmed |
| pubmed-article:11135552 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11135552 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11135552 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11135552 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11135552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11135552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11135552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11135552 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11135552 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11135552 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:11135552 | pubmed:issn | 1087-0156 | lld:pubmed |
| pubmed-article:11135552 | pubmed:author | pubmed-author:NakataniKK | lld:pubmed |
| pubmed-article:11135552 | pubmed:author | pubmed-author:SaitoII | lld:pubmed |
| pubmed-article:11135552 | pubmed:author | pubmed-author:SanduII | lld:pubmed |
| pubmed-article:11135552 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11135552 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:11135552 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11135552 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11135552 | pubmed:pagination | 51-5 | lld:pubmed |
| pubmed-article:11135552 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
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| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:meshHeading | pubmed-meshheading:11135552... | lld:pubmed |
| pubmed-article:11135552 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11135552 | pubmed:articleTitle | Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance. | lld:pubmed |
| pubmed-article:11135552 | pubmed:affiliation | Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan. nakatani@sbchem.kyoto-u.ac.jp | lld:pubmed |
| pubmed-article:11135552 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11135552 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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