| pubmed-article:11122295 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11122295 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:11122295 | lifeskim:mentions | umls-concept:C0022890 | lld:lifeskim |
| pubmed-article:11122295 | lifeskim:mentions | umls-concept:C0004359 | lld:lifeskim |
| pubmed-article:11122295 | lifeskim:mentions | umls-concept:C0443146 | lld:lifeskim |
| pubmed-article:11122295 | lifeskim:mentions | umls-concept:C0005186 | lld:lifeskim |
| pubmed-article:11122295 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:11122295 | pubmed:dateCreated | 2001-2-13 | lld:pubmed |
| pubmed-article:11122295 | pubmed:abstractText | It has been shown that sera from patients with autoimmune inner ear disease contain antibodies to several inner ear antigens. We report here the characterization of the 42-43 kDa protein against which a significant number of patients' sera react strongly. After separation of inner ear proteins from guinea-pig cochleas by SDS-PAGE, the band corresponding to the 42-43 kDa protein was digested with trypsin and the peptide fragments were separated by high-performance liquid chromatography. Two fractions were then subjected to amino acid sequencing by the classical automated Edman degradation. The sequence of a stretch of 15 amino acids of the first fragment was identical to that of amino acids 148-162 of beta-actin. The sequence of the 10 amino acids of the second fragment was also identical to beta-actin. On Western blots, monoclonal antibody directed against beta-actin reacted with the inner ear 42-43 kDa proteins. The serum samples from the patients and the monoclonal antibody reacted with the non-muscle actin used as antigen in Western blotting. Immunoblot analysis of inner ear proteins after two-dimensional gel electrophoresis showed a spot, corresponding to the region of the 43 kDa as compared to the protein standards. On the basis of these data it is concluded that the target 42-43 kDa protein for antibodies in sera of patients with autoimmune inner ear disease is beta-actin, a molecule, which has important and numerous functions inside cells. This is the first report to identify the cytoskeletal protein beta-actin as a candidate autoantigen in autoimmune inner ear disease. | lld:pubmed |
| pubmed-article:11122295 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11122295 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11122295 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11122295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11122295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11122295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11122295 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11122295 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11122295 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11122295 | pubmed:issn | 0307-7772 | lld:pubmed |
| pubmed-article:11122295 | pubmed:author | pubmed-author:GersdorffMM | lld:pubmed |
| pubmed-article:11122295 | pubmed:author | pubmed-author:DeggoujNN | lld:pubmed |
| pubmed-article:11122295 | pubmed:author | pubmed-author:TomasiJ PJP | lld:pubmed |
| pubmed-article:11122295 | pubmed:author | pubmed-author:BoulasselM... | lld:pubmed |
| pubmed-article:11122295 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11122295 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:11122295 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11122295 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11122295 | pubmed:pagination | 535-41 | lld:pubmed |
| pubmed-article:11122295 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:11122295 | pubmed:meshHeading | pubmed-meshheading:11122295... | lld:pubmed |
| pubmed-article:11122295 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11122295 | pubmed:articleTitle | Identification of beta-actin as a candidate autoantigen in autoimmune inner ear disease. | lld:pubmed |
| pubmed-article:11122295 | pubmed:affiliation | Laboratory of Autoimmunity and Department of Otorhinolaryngology, University of Louvain Medical School, 1200 Brussels, Belgium. Rachid.Boulassel@mblg.ucl.ac.be | lld:pubmed |
| pubmed-article:11122295 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11122295 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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