| pubmed-article:11099786 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11099786 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:11099786 | lifeskim:mentions | umls-concept:C0949653 | lld:lifeskim |
| pubmed-article:11099786 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11099786 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11099786 | pubmed:dateCreated | 2001-1-26 | lld:pubmed |
| pubmed-article:11099786 | pubmed:abstractText | In the present study, effects of the alpha(2)- and beta-adrenoceptor agonists clonidine and isoproterenol on astrocytes in astroglial/neuronal cocultures from rat cerebral cortex were evaluated. The calcium- and potassium-sensitive dyes fura-2 and potassium-binding benzofuran isophtalate (PBFI) were used to study alterations in intracellular concentrations of calcium ([Ca(2+)](i)) and potassium ([K(+)](i)), respectively, while the perforated patch clamp technique was used to analyze transmembrane currents. Exposure to isoproterenol or clonidine elicited an immediate increase in [Ca(2+)](i) that was totally abolished in calcium-free extracellular media. Isoproterenol also decreased [K(+)](i), but clonidine did not. The reduction in [K(+)](i) was inhibited in Ca(2+)-free media. As evaluated with the perforated patch technique, isoproterenol (10(-6)-10(-4) M) induced a slowly developing and long lasting outward current that also was totally abolished in calcium-free buffer. This current was blocked by external tetraethylammonium (TEA, 10 mM) and charybdotoxin (ChTX, 10 nM), but was not affected by apamin (50 nM). The current-to-voltage (I-V) relationships for the isoproterenol-induced currents showed a markedly negative reversal potential, -96 mV+/-7, (mean+/-S.D., n=5). These results suggest that the stimulation of astroglial beta-adrenoceptors by isoproterenol opens calcium-activated potassium channels (K((Ca))). Preincubation with forskolin significantly increased the isoproterenol-induced currents compared with controls, indicating that the opening of astroglial K((Ca)) channels after beta-adrenergic stimulation not only depends on [Ca(2+)](i) but also synergistically involves the cAMP transduction system to which beta-adrenoceptors are known to be positively coupled. | lld:pubmed |
| pubmed-article:11099786 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11099786 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11099786 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11099786 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11099786 | pubmed:issn | 0197-0186 | lld:pubmed |
| pubmed-article:11099786 | pubmed:author | pubmed-author:NilssonMM | lld:pubmed |
| pubmed-article:11099786 | pubmed:author | pubmed-author:SinclairJJ | lld:pubmed |
| pubmed-article:11099786 | pubmed:author | pubmed-author:HanssonEE | lld:pubmed |
| pubmed-article:11099786 | pubmed:author | pubmed-author:JardemarkKK | lld:pubmed |
| pubmed-article:11099786 | pubmed:author | pubmed-author:MuydermanHH | lld:pubmed |
| pubmed-article:11099786 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11099786 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:11099786 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11099786 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11099786 | pubmed:pagination | 269-76 | lld:pubmed |
| pubmed-article:11099786 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:11099786 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11099786 | pubmed:articleTitle | Activation of beta-adrenoceptors opens calcium-activated potassium channels in astroglial cells. | lld:pubmed |
| pubmed-article:11099786 | pubmed:affiliation | Institute of Neurobiology and Institute of Clinical Neuroscience, Göteborg University, Box 420, SE-405 30, Göteborg, Sweden. | lld:pubmed |
| pubmed-article:11099786 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11099786 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
