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pubmed-article:11090264pubmed:abstractTextAnisakis simplex, a nematode from the family Anisakidae, is a parasite of fish and mammals. It is a casual agent of a human disease called anisakiosis. We found that the assay based on PCR amplification of the ITS-1-5.8 S-ITS-2 fragment of rDNA and subsequent restriction fragment length polymorphism, previously described on the basis of A. simplex isolated solely from one geographical region, can be used as a general test for identification of this worm species. The restriction patterns analysed for four restriction enzymes were found to be identical in the case of all A. simplex individuals isolated from as different geographical regions as Baltic Sea, Norwegian Sea, Bering Sea and Sea of Okchotsk. Moreover, our results support the previously proposed hypothesis, based on the studies of isoenzymes, that there is a remarkable genetic homogeneity within A. simplex from different geographical regions.lld:pubmed
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pubmed-article:11090264pubmed:authorpubmed-author:RokickiJJlld:pubmed
pubmed-article:11090264pubmed:authorpubmed-author:S?omi?skaMMlld:pubmed
pubmed-article:11090264pubmed:authorpubmed-author:WegrzynGGlld:pubmed
pubmed-article:11090264pubmed:authorpubmed-author:KijewskaAAlld:pubmed
pubmed-article:11090264pubmed:copyrightInfoCopyright 2000 Academic Press.lld:pubmed
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pubmed-article:11090264pubmed:volume14lld:pubmed
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pubmed-article:11090264pubmed:pagination349-54lld:pubmed
pubmed-article:11090264pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11090264pubmed:articleTitleA PCR-RFLP assay for identification of Anisakis simplex from different geographical regions.lld:pubmed
pubmed-article:11090264pubmed:affiliationDepartment of Invertebrate Zoology, University of Gda?sk, Pilsudskiego 46, 81-378 Gdynia, Poland.lld:pubmed
pubmed-article:11090264pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11090264pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed