| pubmed-article:11090148 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0319124 | lld:lifeskim |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0547040 | lld:lifeskim |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0456389 | lld:lifeskim |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0039551 | lld:lifeskim |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:11090148 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:11090148 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:11090148 | pubmed:dateCreated | 2001-1-11 | lld:pubmed |
| pubmed-article:11090148 | pubmed:abstractText | Recombinant adeno-associated virus type 2 (AAV2) can be produced in adenovirus-infected cells by cotransfecting a plasmid containing the recombinant AAV2 genome, which is generally comprised of the viral terminal repeats flanking a transgene, together with a second plasmid expressing the AAV2 rep and cap genes. However, recombinant viruses generally replicate inefficiently, often producing 100-fold fewer virus particles per cell than can be obtained after transfection with a plasmid containing a wild-type AAV2 genome. We demonstrate that this defect is due, at least in part, to the presence of a positive-acting cis element between nucleotides 194 and 1882 of AAV2. Recombinant AAV2 genomes lacking this region accumulated 14-fold less double-stranded, monomer-length replicative-form DNA than did wild-type AAV2. In addition, we demonstrate that a minimum genome size of 3.5 kb is required for efficient production of single-stranded viral DNA. Relatively small recombinant genomes (2,992 and 3,445 bp) accumulated three- to eightfold less single-stranded DNA per monomer-length replicative-form DNA molecule than wild-type AAV2. In contrast, recombinant AAV2 with larger genomes (3,555 to 4,712 bp) accumulated similar amounts of single-stranded DNA per monomer-length replicative-form DNA compared to wild-type AAV2. Analysis of two recombinant AAV2 genomes less than 3.5 kb in size indicated that they were deficient in the production of the extended form of monomer-length replicative-form DNA, which is thought to be the immediate precursor to single-stranded AAV2 DNA. | lld:pubmed |
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| pubmed-article:11090148 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11090148 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11090148 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11090148 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11090148 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11090148 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:11090148 | pubmed:author | pubmed-author:ShenkTT | lld:pubmed |
| pubmed-article:11090148 | pubmed:author | pubmed-author:TullisG EGE | lld:pubmed |
| pubmed-article:11090148 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11090148 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:11090148 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11090148 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11090148 | pubmed:pagination | 11511-21 | lld:pubmed |
| pubmed-article:11090148 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:11090148 | pubmed:meshHeading | pubmed-meshheading:11090148... | lld:pubmed |
| pubmed-article:11090148 | pubmed:meshHeading | pubmed-meshheading:11090148... | lld:pubmed |
| pubmed-article:11090148 | pubmed:meshHeading | pubmed-meshheading:11090148... | lld:pubmed |
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| pubmed-article:11090148 | pubmed:meshHeading | pubmed-meshheading:11090148... | lld:pubmed |
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