| pubmed-article:11074299 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C0225698 | lld:lifeskim |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C1882727 | lld:lifeskim |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C0017037 | lld:lifeskim |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C0702240 | lld:lifeskim |
| pubmed-article:11074299 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:11074299 | pubmed:issue | 1-3 | lld:pubmed |
| pubmed-article:11074299 | pubmed:dateCreated | 2000-12-7 | lld:pubmed |
| pubmed-article:11074299 | pubmed:abstractText | The controlled step in de novo glutathione (GSH) synthesis is catalyzed by gamma-glutamylcysteine synthetase (gamma-GCS), a dimeric enzyme consisting of a heavy catalytic subunit (gamma-GCS-HS) and a light regulatory subunit (gamma-GCS-LS). We have previously reported that exposure to cadmium (Cd) induces pulmonary gamma-GCS-HS mRNA and protein, and that these alterations are accompanied by increases in GSH synthesis and its steady-state level. The current study was designed to test the hypothesis that Cd exposure also up-regulates the expression of the regulatory gamma-GCS subunit. By using northern blotting, we have demonstrated that a single Cd aerosol exposure of adult male Lewis rats results in time- and dose-dependent increases in pulmonary levels of gamma-GCS-LS mRNA. Transcripts of gamma-GCS-LS in rat lung are maximally elevated (8-fold) 2 h following Cd inhalation exposure and remain significantly higher than air controls at 24 h. This response is highly correlated with Cd dose, ranging from 0.9 to 5 mg Cd per m(3), and with lung Cd burden. We also observed Cd-induced up-regulation of gamma-GCS-LS mRNA expression in alveolar epithelial cells exposed to Cd in vitro, either acutely or after repeated passaging in Cd-containing medium. The magnitude of the gamma-GCS regulatory subunit induction observed in Cd-treated cells was approximately five times greater than the induction of the catalytic subunit. These modifications in the expression of gamma-GCS subunits may offer protection from Cd toxicity. | lld:pubmed |
| pubmed-article:11074299 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11074299 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11074299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11074299 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11074299 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11074299 | pubmed:issn | 0300-483X | lld:pubmed |
| pubmed-article:11074299 | pubmed:author | pubmed-author:HartB ABA | lld:pubmed |
| pubmed-article:11074299 | pubmed:author | pubmed-author:ShuklaG SGS | lld:pubmed |
| pubmed-article:11074299 | pubmed:author | pubmed-author:ChiuJJ | lld:pubmed |
| pubmed-article:11074299 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11074299 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:11074299 | pubmed:volume | 151 | lld:pubmed |
| pubmed-article:11074299 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11074299 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11074299 | pubmed:pagination | 45-54 | lld:pubmed |
| pubmed-article:11074299 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11074299 | pubmed:meshHeading | pubmed-meshheading:11074299... | lld:pubmed |
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| pubmed-article:11074299 | pubmed:meshHeading | pubmed-meshheading:11074299... | lld:pubmed |
| pubmed-article:11074299 | pubmed:meshHeading | pubmed-meshheading:11074299... | lld:pubmed |
| pubmed-article:11074299 | pubmed:meshHeading | pubmed-meshheading:11074299... | lld:pubmed |
| pubmed-article:11074299 | pubmed:meshHeading | pubmed-meshheading:11074299... | lld:pubmed |
| pubmed-article:11074299 | pubmed:meshHeading | pubmed-meshheading:11074299... | lld:pubmed |
| pubmed-article:11074299 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11074299 | pubmed:articleTitle | Cadmium-induced elevations in the gene expression of the regulatory subunit of gamma-glutamylcysteine synthetase in rat lung and alveolar epithelial cells. | lld:pubmed |
| pubmed-article:11074299 | pubmed:affiliation | Biochemistry Department, College of Medicine, University of Vermont, Room C-440, Given Medical Building, 05405-0068, Burlington, VT, USA. | lld:pubmed |
| pubmed-article:11074299 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11074299 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11074299 | lld:pubmed |
