pubmed-article:11074009 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C0012984 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C0036849 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C1442518 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C1285573 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C2745888 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C1552652 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C1552685 | lld:lifeskim |
pubmed-article:11074009 | lifeskim:mentions | umls-concept:C1705195 | lld:lifeskim |
pubmed-article:11074009 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:11074009 | pubmed:dateCreated | 2000-11-21 | lld:pubmed |
pubmed-article:11074009 | pubmed:abstractText | One hundred and sixteen interspersed repetitive DNA sequence (IRS)-PCR markers have been developed and characterized from Canis familiaris for high-throughput filter-based genotyping. We present a detailed analysis of markers produced by amplification using primers directed to the conserved regions of the C. familiaris short interspersed nuclear element (Can-SINE). The majority of IRS-PCR markers developed were moderately to highly polymorphic with mean heterozygosity (HET) and polymorphism information content (PIC) values of approximately 0.6. The HET value for 22.3% of the markers exceeded 0.7. We also demonstrate that sequence variation of Can-SINEs between breeds is significant and also represents a rich source of polymorphisms. Mapping of 73 of the markers to the existing integrated linkage-radiation hybrid map enriches the map as well as establishes the utility of the markers. The significance and utility of this new class of IRS-PCR Can-SINE-based markers for high-throughput genotyping is discussed. This method can also be extended to other species that are currently map-poor but have a sufficiently high density of SINEs to allow IRS-PCR. | lld:pubmed |
pubmed-article:11074009 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11074009 | pubmed:language | eng | lld:pubmed |
pubmed-article:11074009 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11074009 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:11074009 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11074009 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:11074009 | pubmed:month | Nov | lld:pubmed |
pubmed-article:11074009 | pubmed:issn | 1531-2267 | lld:pubmed |
pubmed-article:11074009 | pubmed:author | pubmed-author:PelletierJJ | lld:pubmed |
pubmed-article:11074009 | pubmed:author | pubmed-author:DayGG | lld:pubmed |
pubmed-article:11074009 | pubmed:author | pubmed-author:AclandG MGM | lld:pubmed |
pubmed-article:11074009 | pubmed:author | pubmed-author:KoneLL | lld:pubmed |
pubmed-article:11074009 | pubmed:author | pubmed-author:SakuiMM | lld:pubmed |
pubmed-article:11074009 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:11074009 | pubmed:day | 9 | lld:pubmed |
pubmed-article:11074009 | pubmed:volume | 4 | lld:pubmed |
pubmed-article:11074009 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:11074009 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:11074009 | pubmed:pagination | 13-24 | lld:pubmed |
pubmed-article:11074009 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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pubmed-article:11074009 | pubmed:meshHeading | pubmed-meshheading:11074009... | lld:pubmed |
pubmed-article:11074009 | pubmed:year | 2000 | lld:pubmed |
pubmed-article:11074009 | pubmed:articleTitle | A set of canine interrepeat sequence PCR markers for high-throughput genotyping. | lld:pubmed |
pubmed-article:11074009 | pubmed:affiliation | Department of Biochemistry, Department of Oncology, McGill University, Montreal, Quebec, Canada H3G 1Y6. | lld:pubmed |
pubmed-article:11074009 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:11074009 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:11074009 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11074009 | lld:pubmed |