| pubmed-article:11027435 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0546837 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C1709130 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0012860 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0812204 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:11027435 | lifeskim:mentions | umls-concept:C1292733 | lld:lifeskim |
| pubmed-article:11027435 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:11027435 | pubmed:dateCreated | 2000-11-3 | lld:pubmed |
| pubmed-article:11027435 | pubmed:abstractText | Previously, we showed that expression of nm23-H1 is associated inversely with sensitivity to cisplatin in human oesophageal squamous cell carcinoma (OSCC). The present study was undertaken to investigate the association of nm23-H1 expression with cisplatin-induced DNA damage in OSCC using antisense nm23-H1 transfectants. YES-2/AS-12, an antisense nm23-H1-transfected OSCC cell line, showed significantly reduced expression of intracellular nm23-H1 protein compared with that in parental YES-2 cells and YES-2/Neo transfectants. Surface expression of nm23-H1 protein was not observed in any of the three cell lines. PCR analysis for DNA damage demonstrated that YES-2/AS-12 cells were more resistant to nuclear and mitochondrial DNA damage by cisplatin than were YES-2/Neo cells. In addition, mitochondrial membrane potentials and DNA fragmentation assays confirmed that YES-2/AS-12 was more resistant than YES-2/Neo to apoptosis induced by cisplatin. In contrast, YES-2/AS-12 was more sensitive to ouabain, a selective inhibitor of Na(+), K(+)-ATPase, than YES-2 and YES-2/Neo. Pre-treatment with ouabain resulted in no differences in cisplatin sensitivity between the three cell lines examined. Intracellular platinum level in YES-2/AS-12 was significantly lower than that in YES-2 and YES-2/Neo following incubation with cisplatin, whereas ouabain pre-treatment resulted in no differences in intracellular platinum accumulations between the three cell lines. Our data support the conclusion that reduced expression of intracellular nm23-H1 in OSCC cells is associated with cisplatin resistance via the prevention of both nuclear and mitochondrial DNA damage and suggest that it may be related to Na(+), K(+)-ATPase activity, which is responsible for intracellular cisplatin accumulation. | lld:pubmed |
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| pubmed-article:11027435 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11027435 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11027435 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11027435 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11027435 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11027435 | pubmed:issn | 0007-0920 | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:OkiEE | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:HayashiHH | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:MiyamotoKK | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:YoshidaHH | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:HiroseKK | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:YoshinoSS | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:YoshimuraKK | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:HazamaSS | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:TangokuAA | lld:pubmed |
| pubmed-article:11027435 | pubmed:author | pubmed-author:lizukaNN | lld:pubmed |
| pubmed-article:11027435 | pubmed:copyrightInfo | Copyright 2000 Cancer Research Campaign. | lld:pubmed |
| pubmed-article:11027435 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11027435 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:11027435 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11027435 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11027435 | pubmed:pagination | 1209-15 | lld:pubmed |
| pubmed-article:11027435 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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