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pubmed-article:11024458pubmed:abstractTextDalpha3 is a functional alpha-subunit of Drosophila melanogaster nicotinic acetylcholine receptors (nAChRs). Here, we produced Dalpha3-specific antibodies to study which other nAChR subunits can co-assemble with Dalpha3 in receptor complexes of the Drosophila nervous system. Immunohistochemical studies revealed that Dalpha3 is co-distributed with the beta-subunit ARD in synaptic neuropil regions of the optic lobe. Both subunits can be co-purified by alpha-bungarotoxin affinity chromatography. Dalpha3 antibodies co-immunoprecipitate Dalpha3 and ARD proteins and, vice versa, anti-ARD antibodies co-precipitate ARD and Dalpha3. These data demonstrate that one type of fly nAChRs includes these two subunits as integral components.lld:pubmed
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pubmed-article:11024458pubmed:pagination189-92lld:pubmed
pubmed-article:11024458pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:11024458pubmed:articleTitleNeuronal nicotinic acetylcholine receptors of Drosophila melanogaster: the alpha-subunit dalpha3 and the beta-type subunit ARD co-assemble within the same receptor complex.lld:pubmed
pubmed-article:11024458pubmed:affiliationLeibniz Institute for Neurobiology, Department of Neurochemistry and Molecular Biology, Magdeburg, Germany.lld:pubmed
pubmed-article:11024458pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11024458pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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