pubmed-article:11023667 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0383327 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0332152 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
pubmed-article:11023667 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
pubmed-article:11023667 | pubmed:issue | 10 | lld:pubmed |
pubmed-article:11023667 | pubmed:dateCreated | 2000-10-30 | lld:pubmed |
pubmed-article:11023667 | pubmed:abstractText | Interleukin (IL-)18 is an activator of NK cells and a co-inducer of Th(1)cytokines, sharing structural features with the IL-1 family of proteins. Unlike most other cytokines, IL-18 and IL-1beta lack a signal peptide, have an all beta-pleated sheet structure and are synthesized as biologically inactive precursors (pro-IL-18 and pro-IL-1beta). These precursors are cleaved by caspase-1 (IL-1beta-converting enzyme, ICE) to form the biologically active mature cytokines. Direct expression of mature recombinant human IL-18 in E. coli resulted in a partially active cytokine. We tested the possibility that correct folding of huIL-18 requires its prior synthesis as pro-IL-18. Because caspase-1 is not readily available, we constructed an expression vector encoding human pro-IL-18 in which the caspase-1 cleavage site was mutated into a factor Xa site. To facilitate purification, the mutated pro-IL-18 cDNA was fused in frame to a glutathione-S-transferase (GST) coding sequence. The GST-pro-IL-18 fusion protein was expressed in E. coli, captured on glutathione agarose and mature human IL-18, exhibiting high biological activity was released upon cleavage with factor Xa. This result indicates that correct folding of huIL-18 occurs at the level of pro-IL-18 and provides a practical way to produce biologically active huIL-18. | lld:pubmed |
pubmed-article:11023667 | pubmed:language | eng | lld:pubmed |
pubmed-article:11023667 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:11023667 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:11023667 | pubmed:month | Oct | lld:pubmed |
pubmed-article:11023667 | pubmed:issn | 1043-4666 | lld:pubmed |
pubmed-article:11023667 | pubmed:author | pubmed-author:LinKK | lld:pubmed |
pubmed-article:11023667 | pubmed:author | pubmed-author:RubinsteinMM | lld:pubmed |
pubmed-article:11023667 | pubmed:author | pubmed-author:MOER ERE | lld:pubmed |
pubmed-article:11023667 | pubmed:author | pubmed-author:NovickDD | lld:pubmed |
pubmed-article:11023667 | pubmed:copyrightInfo | Copyright 2000 Academic Press. | lld:pubmed |
pubmed-article:11023667 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:11023667 | pubmed:volume | 12 | lld:pubmed |
pubmed-article:11023667 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:11023667 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:11023667 | pubmed:pagination | 1519-25 | lld:pubmed |
pubmed-article:11023667 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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pubmed-article:11023667 | pubmed:year | 2000 | lld:pubmed |
pubmed-article:11023667 | pubmed:articleTitle | Production of a biologically active human interleukin 18 requires its prior synthesis as PRO-IL-18. | lld:pubmed |
pubmed-article:11023667 | pubmed:affiliation | Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel. | lld:pubmed |
pubmed-article:11023667 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:11023667 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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