| pubmed-article:11017873 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11017873 | lifeskim:mentions | umls-concept:C0027747 | lld:lifeskim |
| pubmed-article:11017873 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:11017873 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:11017873 | lifeskim:mentions | umls-concept:C0200765 | lld:lifeskim |
| pubmed-article:11017873 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:11017873 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:11017873 | pubmed:dateCreated | 2000-11-28 | lld:pubmed |
| pubmed-article:11017873 | pubmed:abstractText | We have developed a semi-quantitative method for indirectly revealing variations in the concentration of second messengers (Ca(2+), cyclic AMP) in single presynaptic boutons by detecting the phosphorylation of the synapsins, excellent nerve terminal substrates for cyclic AMP- and Ca(2+)/calmodulin-dependent protein kinases. For this purpose, we employed polyclonal, antipeptide antibodies recognising exclusively synapsin I phosphorylated by Ca(2+)/calmodulin-dependent protein kinase II (at site 3) or synapsins I/II phosphorylated by either cAMP-dependent protein kinase or Ca(2+)/calmodulin-dependent protein kinase I (at site 1). Cerebellar granular neurones in culture were double-labelled with a monoclonal antibody to synapsins I/II and either of the polyclonal antibodies. Digitised images were analysed to determine the relative phosphorylation stoichiometry at each individual nerve terminal. We have found that: (i) under basal conditions, phosphorylation of site 3 was undetectable, whereas site 1 exhibited some degree of constitutive phosphorylation; (ii) depolarisation in the presence of extracellular Ca(2+) was followed by a selective and widespread increase in site 3 phosphorylation, although the relative phosphorylation stoichiometry varied among individual terminals; and (iii) phosphorylation of site 1 was increased by stimulation of cyclic AMP-dependent protein kinase but not by depolarisation and often occurred in specific nerve terminal sub-populations aligned along axon branches. In addition to shedding light on the regulation of synapsin phosphorylation in living nerve terminals, this approach permits the spatially-resolved analysis of the activation of signal transduction pathways in the presynaptic compartment, which is usually too small to be studied with other currently available techniques. | lld:pubmed |
| pubmed-article:11017873 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11017873 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11017873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11017873 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11017873 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:11017873 | pubmed:issn | 0021-9533 | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:GreengardPP | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:CzernikA JAJ | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:BenfenatiFF | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:ValtortaFF | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:DunlapD DDD | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:MenegonAA | lld:pubmed |
| pubmed-article:11017873 | pubmed:author | pubmed-author:CastanoFF | lld:pubmed |
| pubmed-article:11017873 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11017873 | pubmed:volume | 113 ( Pt 20) | lld:pubmed |
| pubmed-article:11017873 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11017873 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11017873 | pubmed:pagination | 3573-82 | lld:pubmed |
| pubmed-article:11017873 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11017873 | pubmed:meshHeading | pubmed-meshheading:11017873... | lld:pubmed |
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| pubmed-article:11017873 | pubmed:meshHeading | pubmed-meshheading:11017873... | lld:pubmed |
| pubmed-article:11017873 | pubmed:meshHeading | pubmed-meshheading:11017873... | lld:pubmed |
| pubmed-article:11017873 | pubmed:meshHeading | pubmed-meshheading:11017873... | lld:pubmed |
| pubmed-article:11017873 | pubmed:meshHeading | pubmed-meshheading:11017873... | lld:pubmed |
| pubmed-article:11017873 | pubmed:meshHeading | pubmed-meshheading:11017873... | lld:pubmed |
| pubmed-article:11017873 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11017873 | pubmed:articleTitle | Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals. | lld:pubmed |
| pubmed-article:11017873 | pubmed:affiliation | Dept Neuroscience, San Raffaele Scientific Institute, Milan, Italy. valtorta.flavia@hsr.it | lld:pubmed |
| pubmed-article:11017873 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11017873 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11017873 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11017873 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11017873 | lld:pubmed |
