| pubmed-article:11011664 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11011664 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:11011664 | lifeskim:mentions | umls-concept:C0009195 | lld:lifeskim |
| pubmed-article:11011664 | lifeskim:mentions | umls-concept:C0600431 | lld:lifeskim |
| pubmed-article:11011664 | lifeskim:mentions | umls-concept:C0034792 | lld:lifeskim |
| pubmed-article:11011664 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:11011664 | pubmed:dateCreated | 2000-10-5 | lld:pubmed |
| pubmed-article:11011664 | pubmed:abstractText | 1. We have investigated the characteristics of the alpha9 acetylcholine receptor (alpha9AChR) expressed in hair cell precursors in an immortalized cell line UB/OC-2 developed from the organ of Corti of the transgenic H-2Kb-tsA58 mouse (the Immortomouse) using both calcium imaging and whole-cell recording. 2. Ratiometric measurements of fura-2 fluorescence revealed an increase of intracellular calcium concentration in cells when challenged with 10 microM ACh. The calcium increase was seen in 66 % of the cells grown at 39 degrees C in differentiated conditions. A sm aller fraction (34%) of cells grown at 33 degrees C in proliferative con ditions responded. 3. Caffeine (10mM) elevated cell calcium. In the ab sence of caffeine, the majority of imaged cells responded only once to A Ch presentations. Pretreatment with caffeine ingibited all calcium respo nses to ACh. 4. In whole-cell tight-seal recordings 10 microM ACh activa ted inward current was dependent on the extracellular calcium concentrat ion with an estimated PCa/PNa of 80 for the alpha9 receptor at physiological calcium levels. 5 . The data indicate that ACh activates a calcium-permeable channel alpha 9AChR in UB/OC-2 cells and that the channel has a significantly higher c alcium permeability than other AChRs. The results indicate that the alp ha9AChR may be able to elevate intracellular calcium levels in hair cell s both directly and via store release. | lld:pubmed |
| pubmed-article:11011664 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11011664 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11011664 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11011664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11011664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11011664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11011664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11011664 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11011664 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11011664 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:11011664 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:11011664 | pubmed:author | pubmed-author:AshmoreJ FJF | lld:pubmed |
| pubmed-article:11011664 | pubmed:author | pubmed-author:RivoltaM NMN | lld:pubmed |
| pubmed-article:11011664 | pubmed:author | pubmed-author:HolleyM CMC | lld:pubmed |
| pubmed-article:11011664 | pubmed:author | pubmed-author:JaggerD JDJ | lld:pubmed |
| pubmed-article:11011664 | pubmed:author | pubmed-author:GriesingerC... | lld:pubmed |
| pubmed-article:11011664 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11011664 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:11011664 | pubmed:volume | 527 Pt 1 | lld:pubmed |
| pubmed-article:11011664 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11011664 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11011664 | pubmed:pagination | 49-54 | lld:pubmed |
| pubmed-article:11011664 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:11011664 | pubmed:meshHeading | pubmed-meshheading:11011664... | lld:pubmed |
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| pubmed-article:11011664 | pubmed:meshHeading | pubmed-meshheading:11011664... | lld:pubmed |
| pubmed-article:11011664 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11011664 | pubmed:articleTitle | Calcium signalling mediated by the 9 acetylcholine receptor in a cochlear cell line from the immortomouse. | lld:pubmed |
| pubmed-article:11011664 | pubmed:affiliation | Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK. | lld:pubmed |
| pubmed-article:11011664 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11011664 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
