| pubmed-article:11000468 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0036125 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0169904 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0332161 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0032150 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C1708111 | lld:lifeskim |
| pubmed-article:11000468 | lifeskim:mentions | umls-concept:C0076206 | lld:lifeskim |
| pubmed-article:11000468 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:11000468 | pubmed:dateCreated | 2000-12-4 | lld:pubmed |
| pubmed-article:11000468 | pubmed:abstractText | One strategy to develop a multi-antigen malaria vaccine is to employ live vectors to carry putative protective Plasmodium falciparum antigens to the immune system. The 19 kDa carboxyl terminus of P. falciparum merozoite surface protein 1 (MSP-1), which is essential for erythrocyte invasion and is a leading antigen for inclusion in a multivalent malaria vaccine, was genetically fused to fragment C of tetanus toxin and expressed within attenuated Salmonella typhi CVD 908. Under conditions in the bacterial cytoplasm, the fragment C-MSP-1 fusion did not form the epidermal growth factor (EGF)-like domains of MSP-1; monoclonal antibodies failed to recognize these conformational domains in immunoblots of non-denatured protein extracted from live vector sonicates. The MSP-1 was nevertheless immunogenic. One month following intranasal immunization of BALB/c mice with the live vector construct, four out of five mice exhibited > or =four-fold rises in anti-MSP-1 by ELISA (GMT=211); a single intranasal booster raised titers further (GMT=1280). Post-immunization sera recognized native MSP-1 on merozoites as determined by indirect immunofluorescence. These data encourage efforts to optimize MSP-1 expression in S. typhi (e.g. as a secreted protein), so that the EGF-like epitopes, presumably necessary for stimulating protective antibodies, can form. | lld:pubmed |
| pubmed-article:11000468 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11000468 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11000468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11000468 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11000468 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:11000468 | pubmed:issn | 0168-1656 | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:HolderA AAA | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:LevineM MMM | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:WuSS | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:SzteinM BMB | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:BeierMM | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:PickettTT | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:GalenJJ | lld:pubmed |
| pubmed-article:11000468 | pubmed:author | pubmed-author:Gómez-DuarteO... | lld:pubmed |
| pubmed-article:11000468 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11000468 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:11000468 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:11000468 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11000468 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11000468 | pubmed:pagination | 125-35 | lld:pubmed |
| pubmed-article:11000468 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:meshHeading | pubmed-meshheading:11000468... | lld:pubmed |
| pubmed-article:11000468 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:11000468 | pubmed:articleTitle | Construction and immunogenicity in mice of attenuated Salmonella typhi expressing Plasmodium falciparum merozoite surface protein 1 (MSP-1) fused to tetanus toxin fragment C. | lld:pubmed |
| pubmed-article:11000468 | pubmed:affiliation | Center for Vaccine Development and the Division of Geographic Medicine, Department of Medicine, University of Maryland, School of Medicine, 685 West Baltimore Street, Baltimore, MD 21201, USA. | lld:pubmed |
| pubmed-article:11000468 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11000468 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11000468 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11000468 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11000468 | lld:pubmed |
