| pubmed-article:10995484 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10995484 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:10995484 | lifeskim:mentions | umls-concept:C0078058 | lld:lifeskim |
| pubmed-article:10995484 | lifeskim:mentions | umls-concept:C0665341 | lld:lifeskim |
| pubmed-article:10995484 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:10995484 | lifeskim:mentions | umls-concept:C1171892 | lld:lifeskim |
| pubmed-article:10995484 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:10995484 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:10995484 | pubmed:dateCreated | 2000-11-3 | lld:pubmed |
| pubmed-article:10995484 | pubmed:abstractText | Vascular endothelial growth factor (VEGF) mediates angiogenic activity in a variety of estrogen target tissues. To determine whether estrogen has a direct transcriptional effect on VEGF gene expression, we developed a model system by transiently transfecting human VEGF promoter-luciferase reporter constructs into primary human endometrial cells and into Ishikawa cells, derived from a well-differentiated human endometrial adenocarcinoma. In primary endometrial epithelial cells, treatment with 17beta-estradiol (E(2)) resulted in a 3.8-fold increase in luciferase activity, whereas a 3. 2-fold induction was demonstrated for stromal cells. Our Ishikawa cells had less than 100 functional estrogen receptors (ER)/cell and were therefore cotransfected with expression vectors encoding either the alpha- or the beta-form of the human ER. In cells cotransfected with ERalpha, E(2) induced 3.2-fold induction in VEGF-promoter luciferase activity. A 2.3-fold increase was observed in cells cotransfected with ERbeta. Through specific deletions, the E(2) response was restricted to a single 385-bp PvuII-SstI fragment in the 5' flanking DNA. Cotransfection of this upstream region with a DNA binding domain ER mutant, or site-directed mutagenesis of a variant ERE within this fragment, resulted in the loss of the E(2) response. Electromobility shift assays demonstrated that this same ERE sequence specifically binds estradiol-ER complexes. These studies demonstrate that E(2)-regulated VEGF gene transcription requires a variant ERE located 1.5 kb upstream from the transcriptional start site. Site-directed mutagenesis of this ERE abrogated E(2)-induced VEGF gene expression. | lld:pubmed |
| pubmed-article:10995484 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10995484 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10995484 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10995484 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10995484 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:10995484 | pubmed:author | pubmed-author:TaylorR NRN | lld:pubmed |
| pubmed-article:10995484 | pubmed:author | pubmed-author:VigneJ LJL | lld:pubmed |
| pubmed-article:10995484 | pubmed:author | pubmed-author:LeitmanD CDC | lld:pubmed |
| pubmed-article:10995484 | pubmed:author | pubmed-author:LebovicD IDI | lld:pubmed |
| pubmed-article:10995484 | pubmed:author | pubmed-author:MuellerM DMD | lld:pubmed |
| pubmed-article:10995484 | pubmed:author | pubmed-author:MinchenkoAA | lld:pubmed |
| pubmed-article:10995484 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10995484 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:10995484 | pubmed:volume | 97 | lld:pubmed |
| pubmed-article:10995484 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10995484 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10995484 | pubmed:pagination | 10972-7 | lld:pubmed |
| pubmed-article:10995484 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:meshHeading | pubmed-meshheading:10995484... | lld:pubmed |
| pubmed-article:10995484 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10995484 | pubmed:articleTitle | Regulation of vascular endothelial growth factor (VEGF) gene transcription by estrogen receptors alpha and beta. | lld:pubmed |
| pubmed-article:10995484 | pubmed:affiliation | Center for Reproductive Sciences, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, CA 94143-0556, USA. | lld:pubmed |
| pubmed-article:10995484 | pubmed:publicationType | Journal Article | lld:pubmed |
