| pubmed-article:10964441 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10964441 | lifeskim:mentions | umls-concept:C0042629 | lld:lifeskim |
| pubmed-article:10964441 | lifeskim:mentions | umls-concept:C0008359 | lld:lifeskim |
| pubmed-article:10964441 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:10964441 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:10964441 | lifeskim:mentions | umls-concept:C1519941 | lld:lifeskim |
| pubmed-article:10964441 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:10964441 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10964441 | pubmed:dateCreated | 2000-10-13 | lld:pubmed |
| pubmed-article:10964441 | pubmed:abstractText | Orochol, a live oral cholera vaccine licensed in Switzerland and in other countries, is based on the genetically modified Vibrio cholerae strain CVD103-HgR. This strain is derived from the wild-type O1 strain Inaba 569B by deletion of a fragment internal to the ctxA gene encoding the A1 subunit of cholera toxin and by replacement of an internal fragment of the hlyA gene with a fragment carrying the mer operon mediating mercury resistance. In this study we describe a polymerase chain reaction (PCR) system for the detection of wild-type Vibrio cholerae and the identification of the vaccine strain for the quality control of production batches. A multiplex PCR system that targets the intact ctxA gene of the wild-type strain and simultaneously the integration site of the mer operon in the hlyA gene (hlyA::mer) of the vaccine strain CVD103-HgR was developed. To evaluate the detection limit of the system, vaccine suspensions were artificially contaminated with wild-type V. cholerae 569B cells and tested by PCR. The detection limit of the system was statistically evaluated and found to be at 11625 wild-type cells per vaccine sachet (95% confidence limit). This number is below the infective dose of wild-type Vibrio cholerae. In Switzerland this test is used in combination with other tests in the official batch-release procedure to assure the safety of each batch of the cholera vaccine Orochol. | lld:pubmed |
| pubmed-article:10964441 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10964441 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10964441 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10964441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10964441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10964441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10964441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10964441 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10964441 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10964441 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10964441 | pubmed:issn | 1045-1056 | lld:pubmed |
| pubmed-article:10964441 | pubmed:author | pubmed-author:StuderEE | lld:pubmed |
| pubmed-article:10964441 | pubmed:author | pubmed-author:CandrianUU | lld:pubmed |
| pubmed-article:10964441 | pubmed:copyrightInfo | Copyright 2000 The International Association for Biologicals. | lld:pubmed |
| pubmed-article:10964441 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10964441 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:10964441 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10964441 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10964441 | pubmed:pagination | 149-54 | lld:pubmed |
| pubmed-article:10964441 | pubmed:dateRevised | 2000-12-18 | lld:pubmed |
| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
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| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
| pubmed-article:10964441 | pubmed:meshHeading | pubmed-meshheading:10964441... | lld:pubmed |
| pubmed-article:10964441 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10964441 | pubmed:articleTitle | Development and validation of a detection system for wild-type Vibrio cholerae in genetically modified cholera vaccine. | lld:pubmed |
| pubmed-article:10964441 | pubmed:affiliation | Official Medicines Control Laboratory Biologika and R&D Unit, Division of Biologicals, Swiss Federal Office of Public Health, Bern, Switzerland. | lld:pubmed |
| pubmed-article:10964441 | pubmed:publicationType | Journal Article | lld:pubmed |
