| pubmed-article:10954531 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10954531 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:10954531 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:10954531 | lifeskim:mentions | umls-concept:C0035380 | lld:lifeskim |
| pubmed-article:10954531 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:10954531 | lifeskim:mentions | umls-concept:C1511545 | lld:lifeskim |
| pubmed-article:10954531 | pubmed:issue | 18 | lld:pubmed |
| pubmed-article:10954531 | pubmed:dateCreated | 2000-9-27 | lld:pubmed |
| pubmed-article:10954531 | pubmed:abstractText | The genome of human immunodeficiency virus type 1 (HIV-1) contains two direct repeats (R) of 97 nucleotides at each end. These elements are of critical importance during the first-strand transfer of reverse transcription, during which the minus-strand strong-stop DNA (-sssDNA) is transferred from the 5' end to the 3' end of the genomic RNA. This transfer is critical for the synthesis of the full-length minus-strand cDNA. These repeats also contain a variety of other functional domains involved in many aspects of the viral life cycle. In this study, we have introduced a series of mutations into the 5', the 3', or both R sequences designed to avoid these other functional domains. Using a single-round infectivity assay, we determined the ability of these mutants to undergo the various steps of reverse transcription utilizing a semiquantitative PCR analysis. We find that mutations within the first 10 nucleotides of either the 5' or the 3' R sequence resulted in virions that were markedly defective for reverse transcription in infected cells. These mutations potentially introduce mismatches between the full-length -sssDNA and 3' acceptor R. Even mutations that would create relatively small mismatches, as little as 3 bp, resulted in inefficient reverse transcription. In contrast, virions containing identically mutated R elements were not defective for reverse transcription or infectivity. Using an endogenous reverse transcription assay with disrupted virus, we show that virions harboring the 5' or the 3' R mutations were not intrinsically defective for DNA synthesis. Similarly sized mismatches slightly further downstream in either the 5', the 3', or both R sequences were not detrimental to continued reverse transcription in infected cells. These data are consistent with the idea that certain mismatches within 10 nucleotides downstream of the U3-R junction in HIV-1 cause defects in the stability of the cDNA before or during the first-strand transfer of reverse transcription leading to the rapid disappearance of the -sssDNA in infected cells. These data also suggest that the great majority of first-strand transfers in HIV-1 occur after the copying of virtually the entire 5' R. | lld:pubmed |
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| pubmed-article:10954531 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10954531 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10954531 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10954531 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10954531 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10954531 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10954531 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:10954531 | pubmed:author | pubmed-author:SAT STS | lld:pubmed |
| pubmed-article:10954531 | pubmed:author | pubmed-author:CleverJ LJL | lld:pubmed |
| pubmed-article:10954531 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10954531 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:10954531 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10954531 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10954531 | pubmed:pagination | 8324-34 | lld:pubmed |
| pubmed-article:10954531 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10954531 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10954531 | pubmed:articleTitle | Sequences in the 5' and 3' R elements of human immunodeficiency virus type 1 critical for efficient reverse transcription. | lld:pubmed |
| pubmed-article:10954531 | pubmed:affiliation | Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA. | lld:pubmed |
| pubmed-article:10954531 | pubmed:publicationType | Journal Article | lld:pubmed |
