| pubmed-article:10952578 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C0010416 | lld:lifeskim |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C0003308 | lld:lifeskim |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C0018437 | lld:lifeskim |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:10952578 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:10952578 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:10952578 | pubmed:dateCreated | 2000-9-25 | lld:pubmed |
| pubmed-article:10952578 | pubmed:abstractText | The Cryptococcus neoformans PMA1 gene, encoding a plasma membrane H(+)-ATPase, was isolated from a genomic DNA library of serotype A strain ATCC 6352. An open reading frame of 3,380 nucleotides contains six introns and encodes a predicted protein consisting of 998 amino acids with a molecular mass of approximately 108 kDa. Plasma membranes were isolated, and the H(+)-ATPase was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be slightly larger than the S. cerevisiae H(+)-ATPase, consistent with its predicted molecular mass. The plasma membrane-bound enzyme exhibited a pH 6.5 optimum for ATP hydrolysis, K(m) and V(max) values of 0.5 mM and 3.1 micromol mg(-1) min(-1), respectively, and an apparent K(i) for vanadate inhibition of 1.6 microM. ATP hydrolysis in plasma membranes and medium acidification by whole cells were inhibited by ebselen, a nonspecific H(+)-ATPase antagonist which was also fungicidal. The predicted C. neoformans protein is 35% identical to proton pumps of both pathogenic and nonpathogenic fungi but exhibits more than 50% identity to PMA1 genes from plants. Collectively, this study provides the basis for establishing the Cryptococcus H(+)-ATPase as a viable target for antifungal drug discovery. | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10952578 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10952578 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10952578 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10952578 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:HuberG KGK | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:VasSS | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:PerlinD SDS | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:SoteropoulosP... | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:SantangeloRR | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:PaderiLL | lld:pubmed |
| pubmed-article:10952578 | pubmed:author | pubmed-author:TamásM JMJ | lld:pubmed |
| pubmed-article:10952578 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10952578 | pubmed:volume | 44 | lld:pubmed |
| pubmed-article:10952578 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10952578 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10952578 | pubmed:pagination | 2349-55 | lld:pubmed |
| pubmed-article:10952578 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:meshHeading | pubmed-meshheading:10952578... | lld:pubmed |
| pubmed-article:10952578 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10952578 | pubmed:articleTitle | Molecular characterization of the plasma membrane H(+)-ATPase, an antifungal target in Cryptococcus neoformans. | lld:pubmed |
| pubmed-article:10952578 | pubmed:affiliation | Public Health Research Institute, New York, NY 10016, USA. | lld:pubmed |
| pubmed-article:10952578 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10952578 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10952578 | lld:pubmed |
