| pubmed-article:10931325 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10931325 | lifeskim:mentions | umls-concept:C0015161 | lld:lifeskim |
| pubmed-article:10931325 | lifeskim:mentions | umls-concept:C0033809 | lld:lifeskim |
| pubmed-article:10931325 | lifeskim:mentions | umls-concept:C1660749 | lld:lifeskim |
| pubmed-article:10931325 | lifeskim:mentions | umls-concept:C1709694 | lld:lifeskim |
| pubmed-article:10931325 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10931325 | pubmed:dateCreated | 2000-10-18 | lld:pubmed |
| pubmed-article:10931325 | pubmed:abstractText | ExoS is a type III cytotoxin of Pseudomonas aeruginosa, which modulates two eukaryotic signalling pathways. The N-terminus (residues 1-234) is a GTPase activating protein (GAP) for RhoGTPases, while the C-terminus (residues 232-453) encodes an ADP-ribosyltransferase. Utilizing a series of N-terminal deletion peptides of ExoS and an epitope-tagged full-length ExoS, two independent domains have been identified within the N-terminus of ExoS that are involved in intracellular localization and expression of GAP activity. N-terminal peptides of ExoS localized to the perinuclear region of CHO cells, and a membrane localization domain was localized between residues 36 and 78 of ExoS. The capacity to elicit CHO cell rounding and express GAP activity resided within residues 90-234 of ExoS, which showed that membrane localization was not required to elicit actin reorganization. ExoS was present in CHO cells as a full-length form, which fractionated with membranes, and as an N-terminally processed fragment, which localized to the cytosol. Thus, ExoS localizes in eukaryotic cells to the perinuclear region and is processed to a soluble fragment, which possesses both the GAP and ADP-ribosyltransferase activities. | lld:pubmed |
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| pubmed-article:10931325 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10931325 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10931325 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10931325 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10931325 | pubmed:issn | 0950-382X | lld:pubmed |
| pubmed-article:10931325 | pubmed:author | pubmed-author:PalSS | lld:pubmed |
| pubmed-article:10931325 | pubmed:author | pubmed-author:FrankD WDW | lld:pubmed |
| pubmed-article:10931325 | pubmed:author | pubmed-author:BarbieriJ TJT | lld:pubmed |
| pubmed-article:10931325 | pubmed:author | pubmed-author:PedersonK JKJ | lld:pubmed |
| pubmed-article:10931325 | pubmed:author | pubmed-author:VallisA JAJ | lld:pubmed |
| pubmed-article:10931325 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10931325 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:10931325 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10931325 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10931325 | pubmed:pagination | 287-99 | lld:pubmed |
| pubmed-article:10931325 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10931325 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10931325 | pubmed:articleTitle | Intracellular localization and processing of Pseudomonas aeruginosa ExoS in eukaryotic cells. | lld:pubmed |
| pubmed-article:10931325 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. | lld:pubmed |
| pubmed-article:10931325 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10931325 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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