| pubmed-article:10923022 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10923022 | lifeskim:mentions | umls-concept:C1516334 | lld:lifeskim |
| pubmed-article:10923022 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:10923022 | lifeskim:mentions | umls-concept:C1518439 | lld:lifeskim |
| pubmed-article:10923022 | lifeskim:mentions | umls-concept:C1622990 | lld:lifeskim |
| pubmed-article:10923022 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:10923022 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:10923022 | pubmed:dateCreated | 2000-9-14 | lld:pubmed |
| pubmed-article:10923022 | pubmed:abstractText | The prp2 gene of fission yeast has previously been shown to encode the large subunit of the splicing factor spU2AF. SpU2AF(59) is an evolutionarily conserved protein that has an arginine/serine-rich region and three RNA recognition motifs (RRMs). We have sequenced three temperature-sensitive alleles of prp2 and determined that the mutations result in single amino acid changes within one of the RRMs or between RRMs. All mutant alleles of prp2 have pre-mRNA splicing defects at the non-permissive temperature. Although the mutant strains are growth-arrested at 37 degrees C, they do not elongate like typical fission yeast cell cycle mutants. The DNA of the prp2(-) strains stains more intensely than a wild-type strain, suggesting that the chromatin may be condensed. Ultrastructural studies show differences in the mutant nuclei including a prominent distinction between the chromatin- and non-chromatin-enriched regions compared to the more homogenous wild-type nucleus. Two-hybrid assays indicate that some of the wild-type protein interactions are altered in the mutant strains. These results suggest that normal functioning of spU2AF(59) may be essential not only for pre-mRNA splicing but also for the maintenance of proper nuclear structure and normal cell cycle progression. | lld:pubmed |
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| pubmed-article:10923022 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10923022 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10923022 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10923022 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10923022 | pubmed:issn | 0749-503X | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:McKinneyRR | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:OhshimaYY | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:TaniTT | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:HabaraYY | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:BealesMM | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:PotashkinJJ | lld:pubmed |
| pubmed-article:10923022 | pubmed:author | pubmed-author:FlaxMM | lld:pubmed |
| pubmed-article:10923022 | pubmed:copyrightInfo | Copyright 2000 John Wiley & Sons, Ltd. | lld:pubmed |
| pubmed-article:10923022 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10923022 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:10923022 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10923022 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10923022 | pubmed:pagination | 1001-13 | lld:pubmed |
| pubmed-article:10923022 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10923022 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10923022 | pubmed:articleTitle | Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure. | lld:pubmed |
| pubmed-article:10923022 | pubmed:affiliation | Department of Cellular and Molecular Pharmacology, Finch University of Health Sciences/The Chicago Medical School, 3333 Green Bay Road, North Chicago, IL 60064, USA. | lld:pubmed |
| pubmed-article:10923022 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10923022 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10923022 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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