| pubmed-article:10897360 | pubmed:abstractText | A purified 200-kDa antigenic fraction from Leishmania donovani axenic amastigotes was evaluated by ELISA for the detection of antibody response in visceral leishmaniasis (VL) patients, post kala-azar dermal leishmaniasis (PKDL) patients and controls, for the diagnosis of visceral leishmaniasis. A positive antibody response to the 200-kDa amastigote fraction and to Leishmania amastigote soluble antigen (LASA) was found in 29 (96.6%) and 30 ((100%) confirmed VL patients, respectively, by the use of ELISA. However, only 1 (10%) out of 10 PKDL patients had detectable antibody response to 200-kDa fraction while all the 10 (100%) PKDL patients exhibited an immune response to LASA. Therefore, use of the 200-kDa antigenic fraction for the detection of antibody response in an ELISA follow-up (post treatment) of VL patients may have prognostic significance, and it may also be useful for differentiating active VL and PKDL. | lld:pubmed |
