| pubmed-article:1084410 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C1185738 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C0020987 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:1084410 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:1084410 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1084410 | pubmed:dateCreated | 1976-9-1 | lld:pubmed |
| pubmed-article:1084410 | pubmed:abstractText | A new method for the isolation of specific immunocompetent lymphocytes has been described in which lymphocyte populations are exposed to fluoresceinated antigens (FLAGs) in vivo or in vitro, and the FLAG-binding cells retained on antifluorescein affinity columns. Specific cells are then eluted with an unrelated FL-labeled protein and shown to be fully immunocompetent. This methodology has been applied successfully in diverse antigenic systems including polymerized flagellin and TNP-specific B cells and alloantigen-reactive cytotoxic T lymphocytes. The method is rapid, inexpensive (requiring only antifluorescein beads), and can be applied to any antigens (or antibodies) in which a fluorescein group can be introduced. | lld:pubmed |
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| pubmed-article:1084410 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1084410 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1084410 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:1084410 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1084410 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1084410 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:1084410 | pubmed:author | pubmed-author:ScottD WDW | lld:pubmed |
| pubmed-article:1084410 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1084410 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:1084410 | pubmed:volume | 144 | lld:pubmed |
| pubmed-article:1084410 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1084410 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1084410 | pubmed:pagination | 69-78 | lld:pubmed |
| pubmed-article:1084410 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1084410 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:1084410 | pubmed:articleTitle | Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro. | lld:pubmed |
| pubmed-article:1084410 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1084410 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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