| pubmed-article:10841773 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0008377 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0028128 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0025255 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0023828 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0178796 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C1996904 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:10841773 | lifeskim:mentions | umls-concept:C1880497 | lld:lifeskim |
| pubmed-article:10841773 | pubmed:issue | 23 | lld:pubmed |
| pubmed-article:10841773 | pubmed:dateCreated | 2000-7-20 | lld:pubmed |
| pubmed-article:10841773 | pubmed:abstractText | The ability of nitric oxide ((*)NO) to inhibit propagative lipid peroxidation was investigated using unilamellar liposomes (LUVs) constituted with egg phosphatidylcholine (PC) or 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), [(14)C]cholesterol (Ch), and a nonregenerable singlet oxygen-derived primer, 5alpha-hydroperoxycholesterol (5alpha-OOH). Exposing LUVs to ascorbate and a lipophilic iron chelate at 37 degrees C resulted in an exponential decay of 5alpha-OOH and accumulation of free radical-derived 7alpha- and 7beta-hydroperoxycholesterol (7alphabeta-OOH), as detected by high-performance liquid chromatography with electrochemical detection. Thiobarbituric acid-reactive species (TBARS) were generated concurrently in egg PC-containing LUVs. Including the (*)NO donor spermine NONOate (SPNO, 5-50 microM) or S-nitroso-N-acetyl-D,L-penicillamine (SNAP, 50-100 microM) in the reaction mixture had no effect on 5alpha-OOH decay (suggesting that iron was not redox-inhibited) but slowed TBARS and 7alphabeta-OOH accumulation in a strongly dose-dependent fashion. Decomposed SPNO or SNAP had no such effects, implying that (*)NO was the responsible agent. Accumulation of several [(14)C]Ch oxidation products, detected by high-performance thin-layer chromatography with phosphorimaging, was similarly diminished by active SPNO or SNAP. Concomitantly, a new band referred to as RCh.4 appeared, the radioactivity of which increased as a function of incubation time and (*)NO donor concentration. RCh.4 material was also generated via direct iron/ascorbate reduction of 7alpha-OOH in the presence of (*)NO, consistent with 7alpha-nitrite (7alpha-ONO) identity. However, various other lines of evidence suggest that RCh.4 is not 7alpha-ONO, but rather 5alpha-hydroxycholesterol (5alpha-OH) generated by reduction of 5alpha-ONO arising from 7alpha-ONO rearrangement. 5alpha-OH was only detected when (*)NO was present in the reaction system, thus providing indirect evidence for the existence of nitrosated Ch intermediates arising from (*)NO chain-breaking activity. | lld:pubmed |
| pubmed-article:10841773 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10841773 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10841773 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10841773 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:10841773 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:10841773 | pubmed:author | pubmed-author:GirottiA WAW | lld:pubmed |
| pubmed-article:10841773 | pubmed:author | pubmed-author:KorytowskiWW | lld:pubmed |
| pubmed-article:10841773 | pubmed:author | pubmed-author:ZarebaMM | lld:pubmed |
| pubmed-article:10841773 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10841773 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:10841773 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:10841773 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10841773 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10841773 | pubmed:pagination | 6918-28 | lld:pubmed |
| pubmed-article:10841773 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:10841773 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10841773 | pubmed:articleTitle | Nitric oxide inhibition of free radical-mediated cholesterol peroxidation in liposomal membranes. | lld:pubmed |
| pubmed-article:10841773 | pubmed:affiliation | Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and Institute of Molecular Biology, Jagiellonian University, Krakow, Poland. | lld:pubmed |
| pubmed-article:10841773 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10841773 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10841773 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
