| pubmed-article:10825549 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C0009325 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C1706515 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C0380603 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C1704640 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C1705192 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C0178587 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:10825549 | lifeskim:mentions | umls-concept:C0331858 | lld:lifeskim |
| pubmed-article:10825549 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:10825549 | pubmed:dateCreated | 2000-9-14 | lld:pubmed |
| pubmed-article:10825549 | pubmed:abstractText | Endothelial cell seeding to improve the performance of small-diameter vascular grafts requires a suitable substrate, such as crosslinked collagen. In addition to providing a suitable substrate for adhesion and growth of endothelial cells, proliferation of seeded endothelial cells can be enhanced by local, sustained release of basic fibroblast growth factor (bFGF, a heparin-binding growth factor for endothelial cells). We have previously shown that collagen crosslinked using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) supports adhesion and proliferation of human umbilical vein endothelial cells (HUVECs). In the present study, HUVECs were seeded on (heparinized) EDC/NHS-crosslinked collagen, pre-loaded with bFGF. Proliferation of HUVECs on (heparinized) crosslinked collagen increased with increasing amounts of pre-loaded bFGF. The minimal cell-seeding density required for proliferation proved to be very low after pre-loading the substrates with bFGF, and was 4-fold lower for heparinized crosslinked collagen compared to crosslinked collagen (250 versus 1000 cells/cm(2)). Pro-coagulant properties (von Willebrand factor secretion and tissue factor expression) of HUVECs seeded on (heparinized) crosslinked collagen, with or without pre-loading of bFGF, were comparable to those of HUVECs on TCPS. It is concluded that heparinized, EDC/NHS-crosslinked collagen pre-loaded with bFGF is a candidate matrix for in vivo endothelial cell seeding of synthetic vascular graft materials. | lld:pubmed |
| pubmed-article:10825549 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10825549 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10825549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10825549 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10825549 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10825549 | pubmed:issn | 0168-3659 | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:van AkenW GWG | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:RohrAA | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:FeijenJJ | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:BeugelingTT | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:EngbersG HGH | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:BeerninkRR | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:ScharenborgN... | lld:pubmed |
| pubmed-article:10825549 | pubmed:author | pubmed-author:WissinkM JMJ | lld:pubmed |
| pubmed-article:10825549 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10825549 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:10825549 | pubmed:volume | 67 | lld:pubmed |
| pubmed-article:10825549 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10825549 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10825549 | pubmed:pagination | 141-55 | lld:pubmed |
| pubmed-article:10825549 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:meshHeading | pubmed-meshheading:10825549... | lld:pubmed |
| pubmed-article:10825549 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10825549 | pubmed:articleTitle | Endothelial cell seeding of (heparinized) collagen matrices: effects of bFGF pre-loading on proliferation (after low density seeding) and pro-coagulant factors. | lld:pubmed |
| pubmed-article:10825549 | pubmed:affiliation | Institute for Biomedical Technology, Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands. | lld:pubmed |
| pubmed-article:10825549 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10825549 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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