| pubmed-article:10816603 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10816603 | lifeskim:mentions | umls-concept:C0004112 | lld:lifeskim |
| pubmed-article:10816603 | lifeskim:mentions | umls-concept:C0014239 | lld:lifeskim |
| pubmed-article:10816603 | lifeskim:mentions | umls-concept:C0456387 | lld:lifeskim |
| pubmed-article:10816603 | lifeskim:mentions | umls-concept:C1698986 | lld:lifeskim |
| pubmed-article:10816603 | lifeskim:mentions | umls-concept:C1514893 | lld:lifeskim |
| pubmed-article:10816603 | lifeskim:mentions | umls-concept:C2699488 | lld:lifeskim |
| pubmed-article:10816603 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10816603 | pubmed:dateCreated | 2000-7-20 | lld:pubmed |
| pubmed-article:10816603 | pubmed:abstractText | Signaling by two classes of endoplasmic reticulum (ER) Ca(2+) stores was studied in primary cultured rat astrocytes. Cytosolic and intra-ER Ca(2+) concentrations ([Ca(2+)](CYT) and [Ca(2+)](ER)) were measured with, respectively, Fura-2 and Furaptra, in separate experiments. The agonists, glutamate and ATP, released Ca(2+) primarily from cyclopiazonic acid (CPA)-sensitive ER Ca(2+) stores (CPA inhibits ER Ca(2+) pumps). Agonist-evoked release was abolished by prior treatment with CPA but was unaffected by prior depletion of caffeine/ryanodine (CAF/RY)-sensitive ER Ca(2+) stores. Conversely, prior depletion of the CPA-sensitive stores did not interfere with Ca(2+) release or reuptake in the CAF/RY-sensitive stores. Unloading of the CPA-sensitive stores, but not the CAF/RY-sensitive stores, promoted Ca(2+) entry through "store-operated channels." Resting [Ca(2+)](ER) averaged 153 microM (based on in situ calibration of Furaptra: K(D) = 76 microM, vs 53 microM in solution). The releasable Ca(2+) in both types of ER Ca(2+) stores was increased by Na(+) pump inhibition with 1 mM ouabain or K(+)-free medium. Using high spatial resolution imaging and image subtraction methods, we observed that some regions of the ER (45-58% of the total ER) unloaded and refilled when CPA was added and removed. Other regions of the ER (24-38%) unloaded and refilled when CAF was added and removed. The overlap between these two classes of ER was only 10-18%. These data indicate that there are two structurally separate, independent components of the ER and that they are responsible for the functional independence of the CPA-sensitive and CAF/RY-sensitive ER Ca(2+) stores. | lld:pubmed |
| pubmed-article:10816603 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10816603 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10816603 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10816603 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10816603 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10816603 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10816603 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10816603 | pubmed:issn | 0894-1491 | lld:pubmed |
| pubmed-article:10816603 | pubmed:author | pubmed-author:BlausteinM... | lld:pubmed |
| pubmed-article:10816603 | pubmed:author | pubmed-author:GolovinaV AVA | lld:pubmed |
| pubmed-article:10816603 | pubmed:copyrightInfo | Copyright 2000 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10816603 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10816603 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:10816603 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10816603 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10816603 | pubmed:pagination | 15-28 | lld:pubmed |
| pubmed-article:10816603 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10816603 | pubmed:meshHeading | pubmed-meshheading:10816603... | lld:pubmed |
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| pubmed-article:10816603 | pubmed:meshHeading | pubmed-meshheading:10816603... | lld:pubmed |
| pubmed-article:10816603 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10816603 | pubmed:articleTitle | Unloading and refilling of two classes of spatially resolved endoplasmic reticulum Ca(2+) stores in astrocytes. | lld:pubmed |
| pubmed-article:10816603 | pubmed:affiliation | Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA. vgolovin@umaryland.edu | lld:pubmed |
| pubmed-article:10816603 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10816603 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10816603 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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