| pubmed-article:10803895 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10803895 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:10803895 | lifeskim:mentions | umls-concept:C1032431 | lld:lifeskim |
| pubmed-article:10803895 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:10803895 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10803895 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:10803895 | lifeskim:mentions | umls-concept:C0173383 | lld:lifeskim |
| pubmed-article:10803895 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10803895 | pubmed:dateCreated | 2000-6-30 | lld:pubmed |
| pubmed-article:10803895 | pubmed:abstractText | Pseudomonas sp. 61-3 accumulated a blend of poly(3-hydroxybutyrate) [P(3HB)] homopolymer and a random copolymer consisting of 3-hydroxyalkanoate (3HA) units of 4-12 carbon atoms. The genes encoding beta-ketothiolase (PhbA(Re)) and NADPH-dependent acetoacetyl-CoA reductase (PhbB(Re)) from Ralstonia eutropha were expressed under the control of promoters for Pseudomonas sp. 61-3 pha locus or R. eutropha phb operon together with phaC1(Ps) gene (PHA synthase 1 gene) from Pseudomonas sp. 61-3 in PHA-negative mutants P. putida GPp104 and R. eutropha PHB(-4) to produce copolyesters [P(3HB-co-3HA)] consisting of 3HB and medium-chain-length 3HA units of 6-12 carbon atoms. The introduction of the three genes into GPp104 strain conferred the ability to synthesize P(3HB-co-3HA) with relatively high 3HB compositions (up to 49 mol%) from gluconate and alkanoates, although 3HB units were not incorporated at all or at a very low fraction (3 mol%) into copolyesters by the strain carrying phaC1Ps gene only. In addition, recombinant strains of R. eutropha PHB(-4) produced P(3HB-co-3HA) with higher 3HB fractions from alkanoates and plant oils than those from recombinant GPp104 strains. One of the recombinant strains, R. eutropha PHB(-4)/ pJKSc46-pha, in which all the genes introduced were expressed under the control of the native promoter for Pseudomonas sp. 61-3 pha locus, accumulated P(3HB-co-3HA) copolyester with a very high 3HB fraction (85 mol%) from palm oil. The nuclear magnetic resonance analyses showed that the copolyesters obtained here were random copolymers of 3HB and 3HA units. | lld:pubmed |
| pubmed-article:10803895 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10803895 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10803895 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10803895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10803895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10803895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10803895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10803895 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10803895 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10803895 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10803895 | pubmed:issn | 0175-7598 | lld:pubmed |
| pubmed-article:10803895 | pubmed:author | pubmed-author:AbeHH | lld:pubmed |
| pubmed-article:10803895 | pubmed:author | pubmed-author:FukuiTT | lld:pubmed |
| pubmed-article:10803895 | pubmed:author | pubmed-author:TaguchiKK | lld:pubmed |
| pubmed-article:10803895 | pubmed:author | pubmed-author:DoiYY | lld:pubmed |
| pubmed-article:10803895 | pubmed:author | pubmed-author:MatsusakiHH | lld:pubmed |
| pubmed-article:10803895 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10803895 | pubmed:volume | 53 | lld:pubmed |
| pubmed-article:10803895 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10803895 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10803895 | pubmed:pagination | 401-9 | lld:pubmed |
| pubmed-article:10803895 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10803895 | pubmed:meshHeading | pubmed-meshheading:10803895... | lld:pubmed |
| pubmed-article:10803895 | pubmed:meshHeading | pubmed-meshheading:10803895... | lld:pubmed |
| pubmed-article:10803895 | pubmed:meshHeading | pubmed-meshheading:10803895... | lld:pubmed |
| pubmed-article:10803895 | pubmed:meshHeading | pubmed-meshheading:10803895... | lld:pubmed |
| pubmed-article:10803895 | pubmed:meshHeading | pubmed-meshheading:10803895... | lld:pubmed |
| pubmed-article:10803895 | pubmed:meshHeading | pubmed-meshheading:10803895... | lld:pubmed |
| pubmed-article:10803895 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10803895 | pubmed:articleTitle | Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant bacteria expressing the PHA synthase gene phaC1 from Pseudomonas sp. 61-3. | lld:pubmed |
| pubmed-article:10803895 | pubmed:affiliation | Polymer Chemistry Laboratory and the RIKEN Group of Japan Science and Technology Corporation, RIKEN Institute, Saitama, Japan. | lld:pubmed |
| pubmed-article:10803895 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10803895 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10803895 | lld:pubmed |
