| pubmed-article:10797571 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10797571 | lifeskim:mentions | umls-concept:C0026239 | lld:lifeskim |
| pubmed-article:10797571 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:10797571 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:10797571 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:10797571 | lifeskim:mentions | umls-concept:C1419366 | lld:lifeskim |
| pubmed-article:10797571 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:10797571 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10797571 | pubmed:dateCreated | 2000-7-10 | lld:pubmed |
| pubmed-article:10797571 | pubmed:abstractText | The effect of Ca(2+)-binding protein regucalcin on Ca(2+)-ATPase activity in isolated rat liver mitochondria was investigated. The presence of regucalcin (0.1, 0.25, and 0.5 microM) in the enzyme reaction mixture led to a significant increase in Ca(2+)-ATPase activity. Regucalcin significantly stimulated ATP-dependent (45)Ca(2+) uptake by the mitochondria. Ruthenium red (10(-5) M) or lanthanum chloride (10(-4) M), an inhibitor of mitochondrial Ca(2+) uptake, completely inhibited regucalcin (0.25 microM)-increased mitochondrial Ca(2+)-ATPase activity and (45)Ca(2+) uptake. The effect of regucalcin (0.25 microM) in increasing Ca(2+)-ATPase activity was completely inhibited by the presence of digitonin (10(-2)%), a solubilizing reagent of membranous lipids, or vanadate (10(-5) M), an inhibitor of phosphorylation of ATPase. The activatory effect of regucalcin (0.25 microM) on Ca(2+)-ATPase activity was not further enhanced in the presence of dithiothreitol (2.5 mM), a protecting reagent of the sulfhydryl (SH) group of the enzyme, or calmodulin (0.60 microM), a modulator protein of Ca(2+) action that could increase mitochondrial Ca(2+)-ATPase activity. The present study demonstrates that regucalcin can stimulate Ca(2+) pump activity in rat liver mitochondria, and that the protein may act on an active site (SH group)-related to phosphorylation of mitochondrial Ca(2+)-ATPase. | lld:pubmed |
| pubmed-article:10797571 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10797571 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10797571 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10797571 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10797571 | pubmed:issn | 0730-2312 | lld:pubmed |
| pubmed-article:10797571 | pubmed:author | pubmed-author:YamaguchiMM | lld:pubmed |
| pubmed-article:10797571 | pubmed:author | pubmed-author:TakahashiHH | lld:pubmed |
| pubmed-article:10797571 | pubmed:copyrightInfo | Copyright 2000 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10797571 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10797571 | pubmed:volume | 78 | lld:pubmed |
| pubmed-article:10797571 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10797571 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10797571 | pubmed:pagination | 121-30 | lld:pubmed |
| pubmed-article:10797571 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:10797571 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10797571 | pubmed:articleTitle | Stimulatory effect of regucalcin on ATP-dependent Ca(2+) uptake activity in rat liver mitochondria. | lld:pubmed |
| pubmed-article:10797571 | pubmed:affiliation | Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Japan. | lld:pubmed |
| pubmed-article:10797571 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10797571 | lld:pubmed |
