| pubmed-article:10770767 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C1262461 | lld:lifeskim |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C0597979 | lld:lifeskim |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C0014406 | lld:lifeskim |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:10770767 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:10770767 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10770767 | pubmed:dateCreated | 2000-5-18 | lld:pubmed |
| pubmed-article:10770767 | pubmed:abstractText | Two ampicillin-resistant (Amp(r)) isolates of Vibrio harveyi, W3B and HB3, were obtained from the coastal waters of the Indonesian island of Java. Strain W3B was isolated from marine water near a shrimp farm in North Java while HB3 was from pristine seawater in South Java. In this study, novel beta-lactamase genes from W3B (bla(VHW-1)) and HB3 (bla(VHH-1)) were cloned and their nucleotide sequences were determined. An open reading frame (ORF) of 870 bp encoding a deduced protein of 290 amino acids (VHW-1) was revealed for the bla gene of strain W3B while an ORF of 849 bp encoding a 283-amino-acid protein (VHH-1) was deduced for bla(VHH-1). At the DNA level, genes for VHW-1 and VHH-1 have a 97% homology, while at the protein level they have a 91% homology of amino acid sequences. Neither gene sequence showed homology to any other beta-lactamases in the databases. The deduced proteins were found to be class A beta-lactamases bearing low levels of homology (<50%) to other beta-lactamases of the same class. The highest level of identity was obtained with beta-lactamases from Pseudomonas aeruginosa, i.e., PSE-1, PSE-4, and CARB-3, and Vibrio cholerae CARB-6. Our study showed that both strains W3B and HB3 possess an endogenous plasmid of approximately 60 kb in size. However, Southern hybridization analysis employing bla(VHW-1) as a gene probe demonstrated that the bla gene was not located in the plasmid. A total of nine ampicillin-resistant V. harveyi strains, including W3B and HB3, were examined by pulsed-field gel electrophoresis of NotI-digested genomic DNA. Despite a high level of intrastrain genetic diversity, the bla(VHW-1) probe hybridized only to an 80- or 160-kb NotI genomic fragment in different isolates. | lld:pubmed |
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| pubmed-article:10770767 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10770767 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10770767 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10770767 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10770767 | pubmed:month | May | lld:pubmed |
| pubmed-article:10770767 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:10770767 | pubmed:author | pubmed-author:HeyV MVM | lld:pubmed |
| pubmed-article:10770767 | pubmed:author | pubmed-author:ROEA MAM | lld:pubmed |
| pubmed-article:10770767 | pubmed:author | pubmed-author:SuwantoAA | lld:pubmed |
| pubmed-article:10770767 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10770767 | pubmed:volume | 44 | lld:pubmed |
| pubmed-article:10770767 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10770767 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10770767 | pubmed:pagination | 1309-14 | lld:pubmed |
| pubmed-article:10770767 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10770767 | pubmed:meshHeading | pubmed-meshheading:10770767... | lld:pubmed |
| pubmed-article:10770767 | pubmed:meshHeading | pubmed-meshheading:10770767... | lld:pubmed |
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| pubmed-article:10770767 | pubmed:meshHeading | pubmed-meshheading:10770767... | lld:pubmed |
| pubmed-article:10770767 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10770767 | pubmed:articleTitle | Novel beta-lactamase genes from two environmental isolates of Vibrio harveyi. | lld:pubmed |
| pubmed-article:10770767 | pubmed:affiliation | Programme in Environmental Microbiology, Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore, Singapore. | lld:pubmed |
| pubmed-article:10770767 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10770767 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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