| pubmed-article:10766345 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0087111 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0206651 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0033262 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0214635 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0808901 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0033713 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C1511636 | lld:lifeskim |
| pubmed-article:10766345 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:10766345 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10766345 | pubmed:dateCreated | 2000-8-4 | lld:pubmed |
| pubmed-article:10766345 | pubmed:abstractText | The cellular oncoprotein Ewing's sarcoma oncogene (EWS)/activating transcription factor 1 (ATF1) is a highly specific marker for malignant melanoma of soft parts (MMSP) and is a potent activator of several cAMP-inducible promoters, including the somatostatin promoter. Here we explored the potential for using the somatostatin promoter to direct toxic gene expression in MMSP cells. When introduced into MMSP cells, a somatostatin-herpes simplex virus thymidine kinase fusion gene confers strong and cell-specific sensitivity to the cytotoxic prodrug ganciclovir. Ganciclovir sensitivity requires the ATF-binding site present in the somatostatin promoter, indicating that toxic gene expression is caused by EWS/ATF1. We also tested the efficacy of recombinant adenoviruses adenoviruses for gene delivery and expression in two MMSP cell lines (DTC1 and Su-ccs-1). Surprisingly, several promoters (including somatostatin) that are strongly activated by EWS/ATF1 in transient assays are not activated in DTC1 and Su-ccs-1 cells when present in an adenovirus vector. In summary, our findings demonstrate the potential for using the somatostatin promoter for cytotoxic prodrug therapy for MMSP. However, first-generation adenovirus vectors cannot be used as promoter delivery vehicles for toxic gene expression in MMSP cells. | lld:pubmed |
| pubmed-article:10766345 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10766345 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10766345 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10766345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10766345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10766345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10766345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10766345 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10766345 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10766345 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10766345 | pubmed:issn | 0929-1903 | lld:pubmed |
| pubmed-article:10766345 | pubmed:author | pubmed-author:LuntR RRR | lld:pubmed |
| pubmed-article:10766345 | pubmed:author | pubmed-author:MenJ LJL | lld:pubmed |
| pubmed-article:10766345 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10766345 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:10766345 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10766345 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10766345 | pubmed:pagination | 396-406 | lld:pubmed |
| pubmed-article:10766345 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:meshHeading | pubmed-meshheading:10766345... | lld:pubmed |
| pubmed-article:10766345 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10766345 | pubmed:articleTitle | The cellular oncogene EWS/activating transcription factor 1 is unable to activate adenovirus-borne promoters: implications for cytotoxic prodrug therapy of malignant melanoma of soft parts. | lld:pubmed |
| pubmed-article:10766345 | pubmed:affiliation | Department of Biology, Hong Kong University of Science and Technology, Kowloon, China. | lld:pubmed |
| pubmed-article:10766345 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10766345 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
