| pubmed-article:10759722 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C0038250 | lld:lifeskim |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C0018957 | lld:lifeskim |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C1550235 | lld:lifeskim |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C0007586 | lld:lifeskim |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C0271510 | lld:lifeskim |
| pubmed-article:10759722 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:10759722 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10759722 | pubmed:dateCreated | 2000-6-2 | lld:pubmed |
| pubmed-article:10759722 | pubmed:abstractText | The objective of this study was to evaluate the cycling status of cord blood (CB)-derived colony-forming cells (CFC) and long-term culture-initiating cells (LTC-IC), and their recruitment into the S-phase of the cell cycle. By using the cytosine arabinoside (Ara-C) suicide approach, we found that only small proportions of both CFC and LTC-IC were in the S-phase of the cell cycle. These estimates were confirmed by flow cytometric DNA analysis, which showed that 96 +/- 2% of CB-derived CD34+ cells were in G0/G1 and only 1.6 +/- 0.4% in the S-phase. Staining of CD34+ cells with an antistatin monoclonal antibody, a marker of the G0 phase, indicated that among CD34+ cells with a flow cytometric DNA content typical of the G0/G1 phase 68 +/- 7% of cells were in the G0 phase of the cell cycle. Incubation (24 h) with interleukin 3 (IL-3), recombinant human stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) significantly increased the proportion of cells in the S-phase for both CFC and LTC-IC without inducing any loss in numbers. Flow cytometric DNA analysis also showed an increase in CD34+ cells in the S-phase upon continuous exposure to these cytokines. Our findings indicate that: (i) very few CB-derived CFC or LTC-IC were in the S-phase of the cell cycle; (ii) a substantial amount of CD34+ cells with a flow cytometric DNA content typical of the G0/G1 fraction was cycling, as found in the G1 phase of the cell cycle; and (iii) 24-h incubation with IL-3, SCF and G-CSF could drive a proportion of progenitor cells into the S-phase without reducing their number. These data might be useful for gene transfer protocols and the ex vivo expansion of CB-derived progenitor cells. | lld:pubmed |
| pubmed-article:10759722 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10759722 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10759722 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10759722 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10759722 | pubmed:issn | 0007-1048 | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:InvernizziRR | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:LucotteGG | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:SalvaneschiLL | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:CazzolaMM | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:TorrettiDD | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:DanovaMM | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:BergamaschiGG | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:RostiVV | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:De AmiciMM | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:PerottiCC | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:PecciAA | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:MalabarbaLL | lld:pubmed |
| pubmed-article:10759722 | pubmed:author | pubmed-author:RamajoliII | lld:pubmed |
| pubmed-article:10759722 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10759722 | pubmed:volume | 108 | lld:pubmed |
| pubmed-article:10759722 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10759722 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10759722 | pubmed:pagination | 621-8 | lld:pubmed |
| pubmed-article:10759722 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:meshHeading | pubmed-meshheading:10759722... | lld:pubmed |
| pubmed-article:10759722 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10759722 | pubmed:articleTitle | Cell cycle distribution of cord blood-derived haematopoietic progenitor cells and their recruitment into the S-phase of the cell cycle. | lld:pubmed |
| pubmed-article:10759722 | pubmed:affiliation | Department of Internal Medicine and Medical Therapy, University of Pavia School of Medicine, Pavia, Italy. | lld:pubmed |
| pubmed-article:10759722 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10759722 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10759722 | lld:pubmed |
