| pubmed-article:10755307 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10755307 | lifeskim:mentions | umls-concept:C0027540 | lld:lifeskim |
| pubmed-article:10755307 | lifeskim:mentions | umls-concept:C0683941 | lld:lifeskim |
| pubmed-article:10755307 | lifeskim:mentions | umls-concept:C0336791 | lld:lifeskim |
| pubmed-article:10755307 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10755307 | pubmed:dateCreated | 2000-6-28 | lld:pubmed |
| pubmed-article:10755307 | pubmed:abstractText | The avirulence genes Avr9 and Avr4 from the fungal tomato pathogen Cladosporium fulvum encode extracellular proteins that elicit a hypersensitive response when injected into leaves of tomato plants carrying the matching resistance genes, Cf-9 and Cf-4, respectively. We successfully expressed both Avr9 and Avr4 genes in tobacco with the Agrobacterium tumefaciens transient transformation assay (agroinfiltration). In addition, we expressed the matching resistance genes, Cf-9 and Cf-4, through agroinfiltration. By combining transient Cf gene expression with either transgenic plants expressing one of the gene partners, Potato virus X (PVX)-mediated Avr gene expression, or elicitor injections, we demonstrated that agroinfiltration is a reliable and versatile tool to study Avr/Cf-mediated recognition. Significantly, agroinfiltration can be used to quantify and compare Avr/Cf-induced responses. Comparison of different Avr/Cf-interactions within one tobacco leaf showed that Avr9/Cf-9-induced necrosis developed slower than necrosis induced by Avr4/Cf-4. Quantitative analysis demonstrated that this temporal difference was due to a difference in Avr gene activities. Transient expression of matching Avr/Cf gene pairs in a number of plant families indicated that the signal transduction pathway required for Avr/Cf-induced responses is conserved within solanaceous species. Most non-solanaceous species did not develop specific Avr/Cf-induced responses. However, co-expression of the Avr4/Cf-4 gene pair in lettuce resulted in necrosis, providing the first proof that a resistance (R) gene can function in a different plant family. | lld:pubmed |
| pubmed-article:10755307 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10755307 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10755307 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10755307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10755307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10755307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10755307 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10755307 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10755307 | pubmed:issn | 0894-0282 | lld:pubmed |
| pubmed-article:10755307 | pubmed:author | pubmed-author:LaurentFF | lld:pubmed |
| pubmed-article:10755307 | pubmed:author | pubmed-author:RothRR | lld:pubmed |
| pubmed-article:10755307 | pubmed:author | pubmed-author:De WitP JPJ | lld:pubmed |
| pubmed-article:10755307 | pubmed:author | pubmed-author:Van der... | lld:pubmed |
| pubmed-article:10755307 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10755307 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:10755307 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10755307 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10755307 | pubmed:pagination | 439-46 | lld:pubmed |
| pubmed-article:10755307 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:10755307 | pubmed:meshHeading | pubmed-meshheading:10755307... | lld:pubmed |
| pubmed-article:10755307 | pubmed:meshHeading | pubmed-meshheading:10755307... | lld:pubmed |
| pubmed-article:10755307 | pubmed:meshHeading | pubmed-meshheading:10755307... | lld:pubmed |
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| pubmed-article:10755307 | pubmed:meshHeading | pubmed-meshheading:10755307... | lld:pubmed |
| pubmed-article:10755307 | pubmed:meshHeading | pubmed-meshheading:10755307... | lld:pubmed |
| pubmed-article:10755307 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10755307 | pubmed:articleTitle | Agroinfiltration is a versatile tool that facilitates comparative analyses of Avr9/Cf-9-induced and Avr4/Cf-4-induced necrosis. | lld:pubmed |
| pubmed-article:10755307 | pubmed:affiliation | Laboratory of Phytopathology, Wageningen University, The Netherlands. | lld:pubmed |
| pubmed-article:10755307 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10755307 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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