| pubmed-article:10745206 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0005220 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0806909 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0178587 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C0162339 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C1550176 | lld:lifeskim |
| pubmed-article:10745206 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:10745206 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10745206 | pubmed:dateCreated | 2000-5-23 | lld:pubmed |
| pubmed-article:10745206 | pubmed:abstractText | Significant improvement in cell growth and protein production has been achieved in Sf-9 insect cell cultures using pulse additions of multicomponent nutrient feed concentrates (Bédard et al., 1994; Chan et al., 1998). The present work focuses on investigating an alternative feeding strategy wherein the nutrients are fed in a semi continuous manner. Fed batch culture experiments were carried out to compare the two different feeding strategies, pulse and semi continuous and a process developed to achieve a cell density of 5.2 x 10(7) cells/mL of Sf-9 cells in a 3.5 L bioreactor. Production of recombinant protein beta-galactosidase was carried out by infecting the cells with baculovirus at a MOI of 10 at cell densities of 17 x 10(6)cells/mL. Specific productivity could be maintained at cell densities as high as 14 x 10(6) cells/mL. The results presented indicate that the feeding method can provide significant improvements in the performance with a reduction in the amount of total nutrients added. On-line monitoring of the culture using the capacitance probe showed that the capacitance probe can be used successfully to monitor the biomass and infection process even at higher cell densities. | lld:pubmed |
| pubmed-article:10745206 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10745206 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10745206 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10745206 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10745206 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10745206 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10745206 | pubmed:month | May | lld:pubmed |
| pubmed-article:10745206 | pubmed:issn | 0006-3592 | lld:pubmed |
| pubmed-article:10745206 | pubmed:author | pubmed-author:BédardCC | lld:pubmed |
| pubmed-article:10745206 | pubmed:author | pubmed-author:KamenA AAA | lld:pubmed |
| pubmed-article:10745206 | pubmed:author | pubmed-author:EliasC BCB | lld:pubmed |
| pubmed-article:10745206 | pubmed:author | pubmed-author:ZeiserAA | lld:pubmed |
| pubmed-article:10745206 | pubmed:copyrightInfo | Copyright 2000 John Wiley & Sons, Inc. | lld:pubmed |
| pubmed-article:10745206 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10745206 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:10745206 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:10745206 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10745206 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10745206 | pubmed:pagination | 381-8 | lld:pubmed |
| pubmed-article:10745206 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:meshHeading | pubmed-meshheading:10745206... | lld:pubmed |
| pubmed-article:10745206 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10745206 | pubmed:articleTitle | Enhanced growth of Sf-9 cells to a maximum density of 5.2 x 10(7) cells per mL and production of beta-galactosidase at high cell density by fed batch culture. | lld:pubmed |
| pubmed-article:10745206 | pubmed:affiliation | Animal Cell Technology and Downstream Processing Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montréal, Québec, Canada, H4P 2R2. | lld:pubmed |
| pubmed-article:10745206 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10745206 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10745206 | lld:pubmed |
