pubmed-article:10742290 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C0026844 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C0006685 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C0080059 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C1150423 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C1268567 | lld:lifeskim |
pubmed-article:10742290 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
pubmed-article:10742290 | pubmed:issue | 7 | lld:pubmed |
pubmed-article:10742290 | pubmed:dateCreated | 2000-5-25 | lld:pubmed |
pubmed-article:10742290 | pubmed:abstractText | 1. Tyrosine kinases have been proposed as regulators of voltage-operated calcium channels. The effects of a range of structurally different inhibitors of protein tyrosine kinases (PTK) were examined on voltage-operated calcium channel currents (I(Ba)) and pp60(c-src) kinase (c-src) activity in vitro. 2. I(Ba) was measured in single myocytes isolated from rabbit ear artery by conventional whole cell voltage-clamp techniques. The activity of purified human c-src was measured in vitro using a non-radioactive assay. 3. Bath application of tyrphostin-23 and genistein (non-selective PTK inhibitors), bistyrphostin (a receptor-PTK-selective inhibitor) and PP1 (a src family-selective inhibitor) inhibited I(Ba) in a concentration-dependent manner over a range of test membrane potentials. Intracellular application of peptide-A, a peptide inhibitor of c-src also inhibited currents. Inhibitor potency series against I(Ba) was PP1 > genistein > tyrphostin 23 > bistyrphostin. 4. Tyrphostin-23, genistein, PP1, and peptide-A shifted the steady-state inactivation curves in a hyperpolarized direction without altering their slope. The inhibitors had no significant effects on I(Ba) activation calculated from current-voltage relationships. 5. The agents inhibited c-src activity in a concentration-dependent manner. The order of potency was PP1 > genistein > peptide-A > tyrphostin-23 > bistyrphostin. The IC(50) for inhibition of c-src activity was similar to the IC(50) for inhibition of I(Ba) in all cases. 6. Western blot analysis with a specific antibody to c-src showed the presence of this cytoplasmic tyrosine kinase in rabbit ear artery cells. 7. A range of structurally dissimilar inhibitors of PTKs inhibit I(Ba) and c-src activity with similar potency. These data provide further evidence implicating endogenous c-src in the modulation of L-type calcium channels in vascular smooth muscle cells. | lld:pubmed |
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pubmed-article:10742290 | pubmed:language | eng | lld:pubmed |
pubmed-article:10742290 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10742290 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:10742290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:10742290 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:10742290 | pubmed:month | Apr | lld:pubmed |
pubmed-article:10742290 | pubmed:issn | 0007-1188 | lld:pubmed |
pubmed-article:10742290 | pubmed:author | pubmed-author:HughesA DAD | lld:pubmed |
pubmed-article:10742290 | pubmed:author | pubmed-author:WijetungeSS | lld:pubmed |
pubmed-article:10742290 | pubmed:author | pubmed-author:LymnJ SJS | lld:pubmed |
pubmed-article:10742290 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:10742290 | pubmed:volume | 129 | lld:pubmed |
pubmed-article:10742290 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:10742290 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:10742290 | pubmed:pagination | 1347-54 | lld:pubmed |
pubmed-article:10742290 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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pubmed-article:10742290 | pubmed:year | 2000 | lld:pubmed |
pubmed-article:10742290 | pubmed:articleTitle | Effects of protein tyrosine kinase inhibitors on voltage-operated calcium channel currents in vascular smooth muscle cells and pp60(c-src) kinase activity. | lld:pubmed |
pubmed-article:10742290 | pubmed:affiliation | Imperial College School of Medicine, National Heart & Lung Institute, St Mary's Hospital, London W2 1NY, UK. v.wijetunge@ic.ac.uk | lld:pubmed |
pubmed-article:10742290 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:10742290 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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