| pubmed-article:10721798 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0206190 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0023828 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0079925 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:10721798 | lifeskim:mentions | umls-concept:C0599896 | lld:lifeskim |
| pubmed-article:10721798 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10721798 | pubmed:dateCreated | 2000-5-11 | lld:pubmed |
| pubmed-article:10721798 | pubmed:abstractText | We have investigated the intracellular fate and antisense effect of oligonucleotide/cationic liposome complexes using phosphorothioate oligonucleotides (S-Oligo) targeted to inducible nitric oxide synthase in mouse peritoneal macrophages. Confocal laser microscopic analysis revealed that, after application of fluorescein isothiocyanate (FITC)-labeled S-Oligo alone, the intracellular localization of fluorescence exhibited a punctate pattern in the cytoplasm, suggesting that the oligonucleotides were mainly confined to the endosomal and/or lysosomal compartments. In the case of complexation with Lipofectin and DMRIE-C liposomes, cellular uptake of FITC-S-Oligo was not greatly enhanced and the fluorescence localization in the cells was similar to that of FITC-S-Oligo alone. LipofectAMINE slightly enhanced cellular uptake of FITC-S-Oligo; however, the intracellular localization profile of FITC-S-Oligo remained largely unchanged. The antisense effect was slightly enhanced by LipofectAMINE under only very limited experimental conditions. It was concluded that cationic liposomes are not a potential carrier for S-Oligo in peritoneal macrophages because of their inability to promote the release of S-Oligo from the endosomal compartments to the cytosol over a non-toxic concentration range. | lld:pubmed |
| pubmed-article:10721798 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10721798 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10721798 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10721798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10721798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10721798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10721798 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10721798 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10721798 | pubmed:issn | 1061-186X | lld:pubmed |
| pubmed-article:10721798 | pubmed:author | pubmed-author:TakagiTT | lld:pubmed |
| pubmed-article:10721798 | pubmed:author | pubmed-author:HashidaMM | lld:pubmed |
| pubmed-article:10721798 | pubmed:author | pubmed-author:YamashitaFF | lld:pubmed |
| pubmed-article:10721798 | pubmed:author | pubmed-author:HashiguchiMM | lld:pubmed |
| pubmed-article:10721798 | pubmed:author | pubmed-author:HiramatsuTT | lld:pubmed |
| pubmed-article:10721798 | pubmed:author | pubmed-author:TakakuraYY | lld:pubmed |
| pubmed-article:10721798 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10721798 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:10721798 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10721798 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10721798 | pubmed:pagination | 363-71 | lld:pubmed |
| pubmed-article:10721798 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:meshHeading | pubmed-meshheading:10721798... | lld:pubmed |
| pubmed-article:10721798 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10721798 | pubmed:articleTitle | Effect of cationic liposomes on intracellular trafficking and efficacy of antisense oligonucleotides in mouse peritoneal macrophages. | lld:pubmed |
| pubmed-article:10721798 | pubmed:affiliation | Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan. | lld:pubmed |
| pubmed-article:10721798 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10721798 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
