| pubmed-article:10701254 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0030274 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0026022 | lld:lifeskim |
| pubmed-article:10701254 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:10701254 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10701254 | pubmed:dateCreated | 2000-4-25 | lld:pubmed |
| pubmed-article:10701254 | pubmed:abstractText | Confocal microscopy with Zinquin, a fluorogenic Zn(2+)-specific indicator, was used for spatially and temporally resolved measurement of Zn2+ efflux from single pancreatic beta-cells. When cells were incubated in buffer containing Zinquin, application of insulin secretagogues evoked an increase in fluorescence around the surface of the cell, indicative of detection of Zn2+ efflux from the cell. The fluorescence increases corresponded spatially and temporally with measurements of exocytosis obtained simultaneously by amperometry. When images were taken at 266-ms intervals, the detection limit for Zn2+ was approximately 0.5 microM. With this image frequency, it was possible to observe bursts of fluorescence which were interpreted as fluctuations of Zn2+ level due to exocytosis. The average intensity of these fluorescence bursts corresponded to a Zn2+ concentration of approximately 7 microM. Since insulin is co-stored with Zn2+ in secretory vesicles, it was concluded that the Zn2+ efflux corresponded to exocytosis of insulin/Zn(2+)-containing granules from the beta-cell. Exocytosis sites identified by this technique were frequently localized to one portion of the cell, indicative of active areas of release. | lld:pubmed |
| pubmed-article:10701254 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10701254 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10701254 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10701254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10701254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10701254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10701254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10701254 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10701254 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10701254 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10701254 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:10701254 | pubmed:author | pubmed-author:KennedyR TRT | lld:pubmed |
| pubmed-article:10701254 | pubmed:author | pubmed-author:AspinwallC... | lld:pubmed |
| pubmed-article:10701254 | pubmed:author | pubmed-author:QianW JWJ | lld:pubmed |
| pubmed-article:10701254 | pubmed:author | pubmed-author:BattisteM AMA | lld:pubmed |
| pubmed-article:10701254 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10701254 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:10701254 | pubmed:volume | 72 | lld:pubmed |
| pubmed-article:10701254 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10701254 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10701254 | pubmed:pagination | 711-7 | lld:pubmed |
| pubmed-article:10701254 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:meshHeading | pubmed-meshheading:10701254... | lld:pubmed |
| pubmed-article:10701254 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10701254 | pubmed:articleTitle | Detection of secretion from single pancreatic beta-cells using extracellular fluorogenic reactions and confocal fluorescence microscopy. | lld:pubmed |
| pubmed-article:10701254 | pubmed:affiliation | Department of Chemistry, University of Florida, Gainesville 32611-7200, USA. | lld:pubmed |
| pubmed-article:10701254 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10701254 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10701254 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10701254 | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10701254 | lld:pubmed |
