| pubmed-article:10694823 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C0080125 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C0038952 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C1332714 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:10694823 | lifeskim:mentions | umls-concept:C0308269 | lld:lifeskim |
| pubmed-article:10694823 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10694823 | pubmed:dateCreated | 2000-3-20 | lld:pubmed |
| pubmed-article:10694823 | pubmed:abstractText | To date, a selective advantage of cells expressing anti-HIV ribozymes has not been shown. This study was undertaken to determine whether such a selective advantage can be demonstrated in vitro. A retroviral vector coding for a hairpin ribozyme targeting the HIV 5'LTR and for the low affinity nerve growth factor receptor (LNGF-RDelta) was designed. Since we demonstrated by RT-PCR that the amount of ribozyme transcripts was highly correlated with the level of surface LNGF-RDelta expression, the vector was utilized to assess ribozyme expression by flow cytometry. Transduced Hut78 and primary CD4+ T cells were purified and subsequently mixed with unmodified cells. After HIV challenge the percentage of ribozyme expressing cells in the cell mixture was monitored by flow cytometry. Twenty-one days after HIV infection the proportion of ribozyme expressing CD4+ T cells was 2.6 times higher in comparison to cells with the control vector. CD4+ T cells with a strong ribozyme expression conferred a 7.4-fold selective advantage at day 21 and a 11.7-fold at day 28. For Hut78 cells a selective advantage was detected exclusively for strongly ribozyme expressing cells. As a mechanism underlying the selective advantage an inhibition of HIV induced apoptosis was shown. These results demonstrate that anti-HIV ribozymes are able to confer a selective survival advantage and indicate that the protective effect is dependent on the amount of ribozyme expression. Gene Therapy (2000) 7, 408-416. | lld:pubmed |
| pubmed-article:10694823 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10694823 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10694823 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10694823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10694823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10694823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10694823 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10694823 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10694823 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:HoelzerDD | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:KleinS ASA | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:PappZZ | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:GrezMM | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:ScherfAA | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:OttmannO GOG | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:EngelsJ WJW | lld:pubmed |
| pubmed-article:10694823 | pubmed:author | pubmed-author:KlebbaCC | lld:pubmed |
| pubmed-article:10694823 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10694823 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:10694823 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10694823 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10694823 | pubmed:pagination | 408-16 | lld:pubmed |
| pubmed-article:10694823 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:meshHeading | pubmed-meshheading:10694823... | lld:pubmed |
| pubmed-article:10694823 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10694823 | pubmed:articleTitle | Retrovirally expressed anti-HIV ribozymes confer a selective survival advantage on CD4+ T cells in vitro. | lld:pubmed |
| pubmed-article:10694823 | pubmed:affiliation | Medizinische Klinik III der Johann Wolfgang Goethe Universität, Frankfurt, Germany. | lld:pubmed |
| pubmed-article:10694823 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10694823 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10694823 | lld:pubmed |
