| pubmed-article:10692221 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C1704387 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C0017287 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C0524892 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C1335439 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C1524075 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:10692221 | lifeskim:mentions | umls-concept:C0337112 | lld:lifeskim |
| pubmed-article:10692221 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10692221 | pubmed:dateCreated | 2000-3-24 | lld:pubmed |
| pubmed-article:10692221 | pubmed:abstractText | Determination of the frequency of mutations at hprt or other loci in human lymphocytes provides a useful biomarker for human exposure to mutagens. One problem, however, is distinguishing between unique mutants and sibling mutants arising as progeny of an earlier mutant cell. We have developed a multiplex polymerase chain reaction (PCR)-based method to analyze T-cell receptor (TCR) gamma gene rearrangements for determination of T-cell clonality in mutational spectrum analysis. PCR primers for different subgroups of the V gene segment of the TCR gamma gene were selected at different sites in the TCR gamma gene so that the size of PCR products could define which V subgroup was involved in rearranged TCR gamma genes; gamma genes involving different V and J subgroups could be determined directly by PCR. Mutant T-lymphocytes with rearranged TCR gamma genes containing the same V and J subgroups were analyzed using PCR-based denaturing polyacrylamide gel electrophoresis. All of the 161 hprt mutant clones analyzed contained rearranged TCR gamma genes. Rearrangements among all subgroups of the V and J gene segments of the TCR gamma gene could be detected. VgammaI and Jgamma1/2 subgroups were involved in 69 and 71% of rearranged TCR gamma genes, respectively. This PCR-based analysis of TCR gamma gene rearrangements provides a simple and comprehensive method for identifying the clonality of mutant T-lymphocytes in human hprt mutant lymphocyte assay and mutational spectrum analysis. | lld:pubmed |
| pubmed-article:10692221 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10692221 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10692221 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10692221 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10692221 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10692221 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10692221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10692221 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10692221 | pubmed:issn | 0893-6692 | lld:pubmed |
| pubmed-article:10692221 | pubmed:author | pubmed-author:SmithD HDH | lld:pubmed |
| pubmed-article:10692221 | pubmed:author | pubmed-author:HsieA WAW | lld:pubmed |
| pubmed-article:10692221 | pubmed:author | pubmed-author:WardJ BJBJr | lld:pubmed |
| pubmed-article:10692221 | pubmed:author | pubmed-author:ILLH MHM | lld:pubmed |
| pubmed-article:10692221 | pubmed:copyrightInfo | Copyright 2000 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10692221 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10692221 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:10692221 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10692221 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10692221 | pubmed:pagination | 1-8 | lld:pubmed |
| pubmed-article:10692221 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:meshHeading | pubmed-meshheading:10692221... | lld:pubmed |
| pubmed-article:10692221 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10692221 | pubmed:articleTitle | Multiplex polymerase chain reaction-based analysis of T-cell receptor gamma gene rearrangements for the determination of T-lymphocyte clonality. | lld:pubmed |
| pubmed-article:10692221 | pubmed:affiliation | Department of Preventive Medicine and Community Health, The University of Texas Medical Branch, Galveston, TX, USA. | lld:pubmed |
| pubmed-article:10692221 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10692221 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10692221 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
