| pubmed-article:10676983 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0006901 | lld:lifeskim |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0302908 | lld:lifeskim |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0025241 | lld:lifeskim |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0596040 | lld:lifeskim |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0599748 | lld:lifeskim |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:10676983 | lifeskim:mentions | umls-concept:C0966503 | lld:lifeskim |
| pubmed-article:10676983 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:10676983 | pubmed:dateCreated | 2000-3-7 | lld:pubmed |
| pubmed-article:10676983 | pubmed:abstractText | Melphalan is a bifunctional alkylating agent that covalently binds with intracellular nucleophilic sites. A methodology using electrospray mass spectrometry was developed to detect and identify DNA adducts. Alkylation sites within a particular nucleotide were examined using electrospray tandem mass spectrometry hyphenated to capillary liquid chromatography in combination with a column switching system. In the reaction mixtures resulting from the interaction of 2'-deoxynucleotides and melphalan several base-aklylated adducts were found. In the case of 2'-deoxyadenosine monophosphate, thymidine monophosphate and 2'-deoxyguanosine phosphate alkylation was observed in the mononucleotide reaction mixtures but not in the DNA-hydrolysates. Calf thymus DNA was reacted in vitro with melphalan. The DNA pellet was isolated and enzymatically hydrolyzed with the aid of Nuclease P1. In this hydrolysate both mono-alkylated 2'-deoxynucleotides and dinucleotides were found. The most important adduct found was identified as the N-7 alklylated dGMP adduct. The alkylated dinucleotides were identified as a pdApdT/melphalan and pdGpdC/melphalan the latter being the most important. | lld:pubmed |
| pubmed-article:10676983 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10676983 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10676983 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10676983 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10676983 | pubmed:issn | 1387-2273 | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:Van DongenWW | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:HoelRR | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:EsmansE LEL | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:BernemanZZ | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:Van den... | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:VanhoutteKK | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:LemièreFF | lld:pubmed |
| pubmed-article:10676983 | pubmed:author | pubmed-author:DeforceDD | lld:pubmed |
| pubmed-article:10676983 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10676983 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:10676983 | pubmed:volume | 736 | lld:pubmed |
| pubmed-article:10676983 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10676983 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10676983 | pubmed:pagination | 43-59 | lld:pubmed |
| pubmed-article:10676983 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:meshHeading | pubmed-meshheading:10676983... | lld:pubmed |
| pubmed-article:10676983 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10676983 | pubmed:articleTitle | Analysis of melphalan adducts of 2'-deoxynucleotides in calf thymus DNA hydrolysates by capillary high-performance liquid chromatography-electrospray tandem mass spectrometry. | lld:pubmed |
| pubmed-article:10676983 | pubmed:affiliation | Department of Chemistry, University of Antwerp, Belgium. | lld:pubmed |
| pubmed-article:10676983 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10676983 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
