| pubmed-article:10666419 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C1267092 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C0002240 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:10666419 | lifeskim:mentions | umls-concept:C1707511 | lld:lifeskim |
| pubmed-article:10666419 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10666419 | pubmed:dateCreated | 2000-2-24 | lld:pubmed |
| pubmed-article:10666419 | pubmed:abstractText | Previous studies have shown that multiple serum response factor (SRF)-binding CArG elements were required for smooth muscle cell (SMC)-specific regulation of smooth muscle (SM) alpha-actin expression. However, a critical question remains as to the mechanisms whereby a ubiquitously expressed transcription factor such as SRF might contribute to SMC-specific expression. The goal of the present study was to investigate the hypothesis that SMC-selective expression of SM alpha-actin is due at least in part to (1) unique CArG flanking sequences that distinguish the SM alpha-actin CArGs from other ubiquitously expressed CArG-dependent genes such as c-fos, (2) cooperative interactions between CArG elements, and (3) SRF-dependent binding of SMC-selective proteins to the CArG-containing regions of the promoter. Results demonstrated that specific sequences flanking CArG B were important for promoter activity in SMCs but not in bovine aortic endothelial cells. We also provided evidence indicating that the structural orientation between CArGs A and B was an important determinant of promoter function. Electrophoretic mobility shift assays and methylation interference footprinting demonstrated that a unique SRF-containing complex formed that was selective for SMCs and, furthermore, that this complex was probably stabilized by protein-protein interactions and not by specific interactions with CArG flanking sequences. Taken together, the results of these studies provide evidence that SM alpha-actin expression in SMCs is complex and may involve the formation of a unique multiprotein initiation complex that is coordinated by SRF complexes bound to multiple CArG elements. | lld:pubmed |
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| pubmed-article:10666419 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10666419 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10666419 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10666419 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10666419 | pubmed:issn | 1524-4571 | lld:pubmed |
| pubmed-article:10666419 | pubmed:author | pubmed-author:OwensG KGK | lld:pubmed |
| pubmed-article:10666419 | pubmed:author | pubmed-author:ThompsonM MMM | lld:pubmed |
| pubmed-article:10666419 | pubmed:author | pubmed-author:Lawrenz-Smith... | lld:pubmed |
| pubmed-article:10666419 | pubmed:author | pubmed-author:MaceC VCV | lld:pubmed |
| pubmed-article:10666419 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:10666419 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:10666419 | pubmed:volume | 86 | lld:pubmed |
| pubmed-article:10666419 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10666419 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10666419 | pubmed:pagination | 221-32 | lld:pubmed |
| pubmed-article:10666419 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10666419 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10666419 | pubmed:articleTitle | Smooth muscle alpha-actin CArG elements coordinate formation of a smooth muscle cell-selective, serum response factor-containing activation complex. | lld:pubmed |
| pubmed-article:10666419 | pubmed:affiliation | Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, USA. | lld:pubmed |
| pubmed-article:10666419 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10666419 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10666419 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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