| pubmed-article:10666126 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0024109 | lld:lifeskim |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0019475 | lld:lifeskim |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0242184 | lld:lifeskim |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10666126 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10666126 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10666126 | pubmed:dateCreated | 2000-3-31 | lld:pubmed |
| pubmed-article:10666126 | pubmed:abstractText | During adaptation to hypoxic and hyperoxic conditions, the genes involved in glucose metabolism are upregulated. To probe involvement of the transcription factor hypoxia-induced factor-1 (HIF-1) in hexokinase (HK) II expression in human pulmonary cells, A549 cells and small-airway epithelial cells (SAECs) were exposed to stimuli such as hypoxia, deferoxamine (DFO), and metal ions. The largest increase in HK-II (20-fold for mRNA and 2.5-fold for enzymatic activity) was observed in A549 cells when exposed to DFO. All stimuli selectively increased the 5.5-kb rather than 4-kb transcript in A549 cells. Cycloheximide and actinomycin D inhibited these responses. In addition, cells were transfected with luciferase reporter constructs driven by the full-length HK-II 5'-regulatory region (4.0 kb) or various deletions of that region. A549 cells transfected with the 4.0-kb construct and exposed to hypoxia or DFO increased their luciferase activity 7- and 10-fold, respectively, indicating that HK-II induction is, at least in part, due to increased gene transcription. Sixty percent of the inducible activity of the 4.0-kb construct was shown to reside within the proximal 0.5 kb. Additionally, cotransfection with a stable HIF-1 mutant and the 4.0-kb promoter construct resulted in increased luciferase activity under normoxic conditions. These results strongly suggest that HK-II is selectively regulated in pulmonary cells by a HIF-1-dependent mechanism. | lld:pubmed |
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| pubmed-article:10666126 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10666126 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10666126 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10666126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10666126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10666126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10666126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10666126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10666126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10666126 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10666126 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10666126 | pubmed:issn | 1040-0605 | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:WhiteC WCW | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:AhmadAA | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:AhmadSS | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:SchneiderB... | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:MalkkiMM | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:DeanS CSC | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:AllenC BCB | lld:pubmed |
| pubmed-article:10666126 | pubmed:author | pubmed-author:RiddleS RSR | lld:pubmed |
| pubmed-article:10666126 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10666126 | pubmed:volume | 278 | lld:pubmed |
| pubmed-article:10666126 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10666126 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10666126 | pubmed:pagination | L407-16 | lld:pubmed |
| pubmed-article:10666126 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10666126 | pubmed:meshHeading | pubmed-meshheading:10666126... | lld:pubmed |
| pubmed-article:10666126 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10666126 | pubmed:articleTitle | Hypoxia induces hexokinase II gene expression in human lung cell line A549. | lld:pubmed |
| pubmed-article:10666126 | pubmed:affiliation | Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA. | lld:pubmed |
| pubmed-article:10666126 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10666126 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10666126 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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