| pubmed-article:10660668 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10660668 | lifeskim:mentions | umls-concept:C0032659 | lld:lifeskim |
| pubmed-article:10660668 | lifeskim:mentions | umls-concept:C0596508 | lld:lifeskim |
| pubmed-article:10660668 | lifeskim:mentions | umls-concept:C0299212 | lld:lifeskim |
| pubmed-article:10660668 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:10660668 | lifeskim:mentions | umls-concept:C0019409 | lld:lifeskim |
| pubmed-article:10660668 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:10660668 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10660668 | pubmed:dateCreated | 2000-5-9 | lld:pubmed |
| pubmed-article:10660668 | pubmed:abstractText | Generation of a floxed Presenilin-1 (PS1) allele involved two recombination events in the embryonic stem (ES) cells. First, a targeting vector containing a loxP site in intron 1 and a floxed CMV-HYG/TK double selection cassette in intron 3 was integrated into the PS1 locus by homologous recombination. The use of a negative selection cassette, PGK-DTA, dramatically increased the recombination efficiency within the targeted locus (75-fold). Second, an expression vector encoding Cre recombinase was introduced to excise the floxed CMV-HYG/TK cassette via site-specific recombination. However, all five ES cell clones testing positive for the proper removal of the CMV-HYG/TK cassette also contained a proportion of ES cells in which recombination had occurred between the distal loxP sites in introns 1 and 3, resulting in excision of the entire floxed region. It is therefore critical to screen for possible recombination events involving all 3 loxP sites, in order to identify ES cells clones bearing high proportions of the desired ES cells. genesis 26:5-8, 2000. | lld:pubmed |
| pubmed-article:10660668 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10660668 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10660668 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10660668 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10660668 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10660668 | pubmed:issn | 1526-954X | lld:pubmed |
| pubmed-article:10660668 | pubmed:author | pubmed-author:YuHH | lld:pubmed |
| pubmed-article:10660668 | pubmed:author | pubmed-author:KesslerJJ | lld:pubmed |
| pubmed-article:10660668 | pubmed:author | pubmed-author:SheaEE | lld:pubmed |
| pubmed-article:10660668 | pubmed:copyrightInfo | Copyright 2000 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10660668 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10660668 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:10660668 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10660668 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10660668 | pubmed:pagination | 5-8 | lld:pubmed |
| pubmed-article:10660668 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10660668 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10660668 | pubmed:articleTitle | Heterogeneous populations of ES cells in the generation of a floxed Presenilin-1 allele. | lld:pubmed |
| pubmed-article:10660668 | pubmed:affiliation | Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. | lld:pubmed |
| pubmed-article:10660668 | pubmed:publicationType | Journal Article | lld:pubmed |
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