| pubmed-article:10655385 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C1552137 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0012933 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0004610 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0597356 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0600597 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0332283 | lld:lifeskim |
| pubmed-article:10655385 | lifeskim:mentions | umls-concept:C0175671 | lld:lifeskim |
| pubmed-article:10655385 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10655385 | pubmed:dateCreated | 2000-3-16 | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:abstractText | The rapid identification of bacteria in blood cultures and other clinical specimens is important for patient management and antimicrobial therapy. We describe a rapid (<4 h) detection and identification system that uses universal PCR primers to amplify a variable region of bacterial 23S ribosomal DNA, followed by reverse hybridization of the products to a panel of oligonucleotides. This procedure was successful in discriminating a range of bacteria in pure cultures. When this procedure was applied directly to 158 unselected positive blood culture broths on the day when growth was detected, 125 (79.7%) were correctly identified, including 4 with mixed cultures. Nine (7.2%) yielded bacteria for which no oligonucleotide targets were present in the oligonucleotide panel, and 16 culture-positive broths (10.3%) produced no PCR product. In seven of the remaining eight broths, streptococci were identified but not subsequently grown, and one isolate of Staphylococcus aureus was misidentified as a coagulase-negative staphylococcus. The accuracy, range, and discriminatory power of the assay can be continually extended by adding further oligonucleotides to the panel without significantly increasing complexity or cost. | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10655385 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10655385 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10655385 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10655385 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10655385 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:10655385 | pubmed:author | pubmed-author:AnthonyR MRM | lld:pubmed |
| pubmed-article:10655385 | pubmed:author | pubmed-author:BrownT JTJ | lld:pubmed |
| pubmed-article:10655385 | pubmed:author | pubmed-author:FrenchG LGL | lld:pubmed |
| pubmed-article:10655385 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10655385 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:10655385 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10655385 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10655385 | pubmed:pagination | 781-8 | lld:pubmed |
| pubmed-article:10655385 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:meshHeading | pubmed-meshheading:10655385... | lld:pubmed |
| pubmed-article:10655385 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10655385 | pubmed:articleTitle | Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. | lld:pubmed |
| pubmed-article:10655385 | pubmed:affiliation | Department of Microbiology, King's College St. Thomas' Campus, St. Thomas' Hospital, London SE1 7EH, United Kingdom. | lld:pubmed |
| pubmed-article:10655385 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10655385 | lld:pubmed |
